Activity, diversity and population size of ammonia-oxidising bacteria in oil-contaminated landfarming soil

• Published in: FEMS microbiology letters Vol. 250; no. 1; pp. 33 - 38
• Main Authors: Kurola, Jukka, Salkinoja-Salonen, Mirja, Aarnio, Tuula, Hultman, Jenni, Romantschuk, Martin
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 01.09.2005; Blackwell Publishing Ltd; Blackwell; Oxford University Press
• Subjects: Agronomy. Soil science and plant productions; Ammonia; Ammonia - metabolism; Ammonium; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bacteria - metabolism; Bacteriology; Biochemistry and biology; Biological and medical sciences; Chemical, physicochemical, biochemical and biological properties; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; Electrophoresis; Fundamental and applied biological sciences. Psychology; Gel electrophoresis; Gene sequencing; Genes, Bacterial; Genetic diversity; Genetic Variation; Growing season; Metabolism. Enzymes; Microbiology; Nitrogen dioxide; Nitrosomonadaceae - classification; Nitrosomonadaceae - genetics; Nitrosomonadaceae - isolation & purification; Nitrosomonadaceae - metabolism; Nitrosospira sp; Oil pollution; Oils - metabolism; Oxidation; Oxidation-Reduction; Phylogeny; Physics, chemistry, biochemistry and biology of agricultural and forest soils; Polymerase chain reaction; Population number; Real-time PCR; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; rRNA 16S; Soil bacteria; Soil contamination; Soil Microbiology; Soil microorganisms; Soil Pollutants - metabolism; Soil pollution; Soil science; Soils; Terrestrial environments; Genetic diversity; Real-time PCR; Nitrosospira sp; Soil contamination; sp; Nitrobacteraceae; Phylogenetic tree; Denaturing gradient gel electrophoresis; Real time; Contamination; Polymerase chain reaction; Soils; Bacteria; Nitrosospira; Sequencing
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/j.femsle.2005.06.057

Chemolithotrophic ammonia-oxidising bacteria (AOB) present in oil-contaminated landfarming soil were studied over two growing seasons in 1999 and 2000. The number of AOB (4–9 × 10 5 cells g −1 of dry soil) determined with the quantitative polymerase chain reaction (real-time PCR) and the rate of potential ammonium oxidation ( 0 . 05 – 0 . 28 μ g NO 2 - – N g - 1 of dry soil h - 1 ) indicated the presence of stable AOB populations. Denaturing gradient gel electrophoresis (DGGE) profiling and sequence analysis of PCR-amplified AOB 16S rRNA genes showed dominance of Nitrosospira-like sequences in clusters 2 and 3. The present results from the chronically oil-contaminated landfarming soil support the suggested importance of Nitrosospira-like AOB in terrestrial environments.