Activity, diversity and population size of ammonia-oxidising bacteria in oil-contaminated landfarming soil
Chemolithotrophic ammonia-oxidising bacteria (AOB) present in oil-contaminated landfarming soil were studied over two growing seasons in 1999 and 2000. The number of AOB (4–9 × 10 5 cells g −1 of dry soil) determined with the quantitative polymerase chain reaction (real-time PCR) and the rate of pot...
Saved in:
Published in | FEMS microbiology letters Vol. 250; no. 1; pp. 33 - 38 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Elsevier B.V
01.09.2005
Blackwell Publishing Ltd Blackwell Oxford University Press |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Chemolithotrophic ammonia-oxidising bacteria (AOB) present in oil-contaminated landfarming soil were studied over two growing seasons in 1999 and 2000. The number of AOB (4–9
×
10
5
cells
g
−1 of dry soil) determined with the quantitative polymerase chain reaction (real-time PCR) and the rate of potential ammonium oxidation
(
0
.
05
–
0
.
28
μ
g
NO
2
-
–
N
g
-
1
of
dry
soil
h
-
1
)
indicated the presence of stable AOB populations. Denaturing gradient gel electrophoresis (DGGE) profiling and sequence analysis of PCR-amplified AOB 16S rRNA genes showed dominance of
Nitrosospira-like sequences in clusters 2 and 3. The present results from the chronically oil-contaminated landfarming soil support the suggested importance of
Nitrosospira-like AOB in terrestrial environments. |
---|---|
Bibliography: | Edited by E. Baggs ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0378-1097 1574-6968 |
DOI: | 10.1016/j.femsle.2005.06.057 |