Non-invasive sampling technique for DNA extraction from captive Japanese Crested Ibis on Sado Island
The Japanese Crested Ibis Nipponia nippon is a critically threatened bird. The post-hatch eggs of the current captive population of this species on Sado Island have been stored at room temperature for the long-term. In this study, we investigated the suitability of the vascularized chorioallantois m...
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Published in | Animal science journal Vol. 82; no. 4; pp. 616 - 619 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Melbourne, Australia
Blackwell Publishing Asia
01.08.2011
Blackwell Publishing Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | The Japanese Crested Ibis Nipponia nippon is a critically threatened bird. The post-hatch eggs of the current captive population of this species on Sado Island have been stored at room temperature for the long-term. In this study, we investigated the suitability of the vascularized chorioallantois membrane from the eggs as a non-invasive DNA source. Using microsatellite loci developed for the Japanese Crested Ibis, we performed three experiments for comparison of genotypes obtained among DNA. First, DNA from five different sites of the identical membrane showed the same genotypes at either of two loci examined. Second, DNA from the membrane of each full-sibling birds and blood of their parents showed the genotypes that were consistent with Mendelian parent-offspring relationships at any of eight loci examined. Third, DNA from the membrane and blood of the same bird showed the matched genotypes at any of eight loci examined. These results indicate that the vascularized chorioallantois membrane from post-hatch eggs stored at room temperature for the long- term can be used as a reliable DNA source of offspring that had hatched from the egg. This study will promote a molecular genetics study on genetic diversity of the current captive Japanese Crested Ibis population on Sado Island. |
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Bibliography: | http://dx.doi.org/10.1111/j.1740-0929.2011.00902.x istex:694D6DBC71FF9C95BC63D0762C936B2E81723A5A ArticleID:ASJ902 ark:/67375/WNG-0RG49P3C-0 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 1344-3941 1740-0929 |
DOI: | 10.1111/j.1740-0929.2011.00902.x |