沙田柚RING-H2 finger基因的生物信息学分析

为探讨沙田柚自交不亲和性的机理，利用高通量测序技术对沙田柚自交和异交花柱进行转录组测序，通过差异分析得到了沙田柚RING—H2finger基因序列。采用生物信息学方法，对其编码的蛋白质从序列特征、理化性质、跨膜结构域、高级结构以及功能域等方面进行了预测和分析。结果表明：该基因全长为845bp，开放阅读框（ORF）全长为387bp，编码128个氨基酸，编码蛋白质的分子量为13．61KDa，理论等电点为4．26；该蛋白质含有一个植物RING-H2finger蛋白家族的保守结构域，为疏水性不稳定蛋白。氨基酸序列分析表明，其编码的氨基酸与甜橙（Cs4g15340．1）RING—H2finger蛋白的相...

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Bibliographic Details
Published in生物信息学 Vol. 15; no. 3; pp. 149 - 155
Main Author 李惠敏;罗琼;肖君;李少梅;刘华英;秦新民
Format Journal Article
LanguageChinese
Published 广西师范大学 生命科学学院,广西 桂林,541004 2017
Subjects
finger蛋白基因
RING-H2
沙田柚
环指结构域
生物信息学
生物信息学
Citrus grandis var. Shatinyu Hort
沙田柚
RING?H2 finger protein gene
bioinformatics
RING finger domain
环指结构域
RING?H2finger蛋白基因
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ISSN1672-5565
DOI10.3969/j.issn.1672-5565.201701005

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Summary:为探讨沙田柚自交不亲和性的机理，利用高通量测序技术对沙田柚自交和异交花柱进行转录组测序，通过差异分析得到了沙田柚RING—H2finger基因序列。采用生物信息学方法，对其编码的蛋白质从序列特征、理化性质、跨膜结构域、高级结构以及功能域等方面进行了预测和分析。结果表明：该基因全长为845bp，开放阅读框（ORF）全长为387bp，编码128个氨基酸，编码蛋白质的分子量为13．61KDa，理论等电点为4．26；该蛋白质含有一个植物RING-H2finger蛋白家族的保守结构域，为疏水性不稳定蛋白。氨基酸序列分析表明，其编码的氨基酸与甜橙（Cs4g15340．1）RING—H2finger蛋白的相似度为98％。这些分析结果可为今后深入研究该蛋白的结构特征和功能提供参考。
Bibliography:Citrus grandis var. Shatinyu Hort; RING-H2 finger protein gene; bioinformatics;RING finger domain
23-1513/Q
To explore the molecular mechanism of self-incompatibility, the transcriptomes of the self-pollinated style and cross-pollinated style of Citrus grandis var. Shatinyu Hort were sequenced by high-throughput sequencing technology. RING-H2 finger gene sequence of Citrus grandis var. Shatinyu Hort was obtained through differential analysis method, and some characters of the RING-H2 finger protein were analyzed and predicted by bioinformatics method, including the composition of amino acid sequence, physicochemical parameters, hydrophobicity, transmembrane domain, structure and function of protein etc. The results showed that RING-H2 finger gene was 845 bp in length with an open reading frame（ORF） of 387 bp, encoding 128 amino acids with deduced molecular weight of 13.61 KDa, and theoretical pI value of 4.26, and contained a RING-H2 finger domain. Bioinformatics analysis showed that RING-H2 finger protein was
ISSN:1672-5565
DOI:10.3969/j.issn.1672-5565.201701005