低温对香樟膜脂过氧化和保护酶活性的影响

以徐州引种驯化的成年香樟树叶片为材料,采用模拟低温胁迫法（温度处理分别为0℃、-5℃、-10℃、-15℃、-20℃、-25℃）,研究了低温对成年香樟膜脂过氧化反应和保护酶活性的影响。结果显示,随着处理温度的降低,其叶片细胞质膜相对透性呈＂S＂型曲线增大,用Logistic方程计算其半致死温度为-13.40℃。叶片中MDA不断增加,SOD活性、POD活性、游离脯氨酸含量及可溶性糖含量随着处理温度的降低,呈先升后降的变化趋势。...

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Bibliographic Details
Published in广东农业科学 Vol. 38; no. 4; pp. 58 - 60
Main Author 孙存华 孙存玉 张亚红 施剑杰 孙东 李百伟 王丹 蒋继宏
Format Journal Article
LanguageChinese
Published 江苏省药用植物生物技术重点实验室,江苏徐州221116%徐州师范大学生命科学学院,江苏徐州221116 2011
睢宁县林业指导站,江苏睢宁221200%徐州师范大学生命科学学院,江苏徐州,221116%江苏省药用植物生物技术重点实验室,江苏徐州,221116
徐州师范大学生命科学学院,江苏徐州221116
Subjects
低温胁迫
生理生化反应
香樟
生理生化反应
香樟
低温胁迫
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ISSN1004-874X
DOI10.3969/j.issn.1004-874X.2011.04.023

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Summary:以徐州引种驯化的成年香樟树叶片为材料,采用模拟低温胁迫法（温度处理分别为0℃、-5℃、-10℃、-15℃、-20℃、-25℃）,研究了低温对成年香樟膜脂过氧化反应和保护酶活性的影响。结果显示,随着处理温度的降低,其叶片细胞质膜相对透性呈＂S＂型曲线增大,用Logistic方程计算其半致死温度为-13.40℃。叶片中MDA不断增加,SOD活性、POD活性、游离脯氨酸含量及可溶性糖含量随着处理温度的降低,呈先升后降的变化趋势。
Bibliography:44-1267/S
SUN Cun-hua,SUN Cun-yu,ZHANG Ya-hong,SHI Jian-jie,SUN Dong, LI Bai-wei,WANG Dan,JIANG Ji-hong（1.College of Life Sciences,Xuzhou normal university,Xuzhou 221116,China;2.Key Lab of Biotech ndogy for Medicinal Plants of Jiangsu Province,Xuzhou 221116,China;3.Guiding Station of Suining County,Suining 221200,China）
low temperature stress; Cinnamomum camphora; physiologial and biochemicial response
The effect of low temperature stress on membrane lipid peroxidation and protective enzyme activity in leaves of Cinnamomum camphora were studied with simulation low-temperature stress method（testing temperature was dropped from 0℃ to-25℃ gradually）in this paper.The results showed that the plasma membrane relative permeability in leaves of Cinnamomum camphora increased like a S-curve as the decreasing of temperature.Logistic equation showed that the semilethal temperature was-13.40℃.The content of MDA in the leaves increased,whereas proline,soluble sugar,the activity of SOD and POD in the leaves increased at first
ISSN:1004-874X
DOI:10.3969/j.issn.1004-874X.2011.04.023