5-aza-dc和TSA对广西陆川猪体细胞核移植的影响
为探讨甲基转移酶抑制剂5-aza-dc、去乙酰基转移酶抑制剂TSA对广西陆川猪体细胞核移植效率的影响,用5-aza-dc、TSA处理供体细胞和重构胚。结果显示,5-Aza-dc联合TSA处理供体细胞、重构胚以及连续处理供体细胞和重构胚,桑椹胚率均显著高于对照组（P〈0.05）,但各组间囊胚率差异不显著。说明5-aza-dc联合TSA处理供体细胞和克隆胚能提高猪克隆胚的发育能力,尽管差异不显著。...
Saved in:
| Published in | 西南农业学报 Vol. 24; no. 6; pp. 2372 - 2375 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
亚热带农业生物资源保护与利用国家重点实验室/广西大学动物繁殖研究所,广西南宁,530004
2011
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | 为探讨甲基转移酶抑制剂5-aza-dc、去乙酰基转移酶抑制剂TSA对广西陆川猪体细胞核移植效率的影响,用5-aza-dc、TSA处理供体细胞和重构胚。结果显示,5-Aza-dc联合TSA处理供体细胞、重构胚以及连续处理供体细胞和重构胚,桑椹胚率均显著高于对照组（P〈0.05）,但各组间囊胚率差异不显著。说明5-aza-dc联合TSA处理供体细胞和克隆胚能提高猪克隆胚的发育能力,尽管差异不显著。 |
|---|---|
| AbstractList | S828; 为探讨甲基转移酶抑制剂5-aza-dc、去乙酰基转移酶抑制剂TSA对广西陆川猪体细胞核移植效率的影响,用5-aza-dc、TSA处理供体细胞和重构胚.结果显示,5 -Aza-dc联合TSA处理供体细胞、重构胚以及连续处理供体细胞和重构胚,桑椹胚率均显著高于对照组(P＜0.05),但各组间震胚率差异不显著.说明5 -aza-dc联合TSA处理供体细胞和克隆胚能提高猪克隆胚的发育能力,尽管差异不显著. 为探讨甲基转移酶抑制剂5-aza-dc、去乙酰基转移酶抑制剂TSA对广西陆川猪体细胞核移植效率的影响,用5-aza-dc、TSA处理供体细胞和重构胚。结果显示,5-Aza-dc联合TSA处理供体细胞、重构胚以及连续处理供体细胞和重构胚,桑椹胚率均显著高于对照组（P〈0.05）,但各组间囊胚率差异不显著。说明5-aza-dc联合TSA处理供体细胞和克隆胚能提高猪克隆胚的发育能力,尽管差异不显著。 |
| Author | 秦小娥 王猛 宋雪梅 卢晟盛 卢克焕 |
| AuthorAffiliation | 亚热带农业生物资源保护与利用国家重点实验室／广西大学动物繁殖研究所,广西南宁530004 |
| AuthorAffiliation_xml | – name: 亚热带农业生物资源保护与利用国家重点实验室/广西大学动物繁殖研究所,广西南宁,530004 |
| Author_FL | QIN Xiao-e LU Ke-huan SONG Xue-mei LU Sheng-sheng WANG Meng |
| Author_FL_xml | – sequence: 1 fullname: QIN Xiao-e – sequence: 2 fullname: WANG Meng – sequence: 3 fullname: SONG Xue-mei – sequence: 4 fullname: LU Sheng-sheng – sequence: 5 fullname: LU Ke-huan |
| Author_xml | – sequence: 1 fullname: 秦小娥 王猛 宋雪梅 卢晟盛 卢克焕 |
| BookMark | eNo9j79Lw0AcxW-oYK39IwTBKfF7d7nLHU6l-AsKDmYvl2tSU_SiDWLrqnRqFzspKA6K4CJOWkrpP2Oa-l8YqTg93uPDe7wVVDCxCRBax2BTyeVmy46SxNgYAFuOINImgLEN3AaXFlDxP19G5SSJfABCOKWMFNEWs9Slsho6vel7h5X0bZSOpvPn6fdtL_24z_qvX5NhNu7Nrx5mj5_Zy3j2NMjurtPJezocrKKlUB0nQflPS8jb2faqe1btYHe_WqlZmglqYQY8EDyQypWKKZwbxkPNhYNlg_qhJgEVVIN0taANIoBr35eO68qQ-Jw7tIQ2FrUXyoTKNOut-Lxt8sF6x5hux__9Cjx_mpNrC1IfxaZ5FuXsaTs6Ue1u3QEmHJdg-gOwbGlG |
| ClassificationCodes | S828 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W95 ~WA 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.3969/j.issn.1001-4829.2011.06.073 |
| DatabaseName | 维普_期刊 中文科技期刊数据库-CALIS站点 维普中文期刊数据库 中文科技期刊数据库-农业科学 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| DocumentTitleAlternate | Effect of 5-aza-dc and TSA on Somatic Nuclear Transfer of Guangxi Luchuan-pig |
| DocumentTitle_FL | Effect of 5-aza-dc and TSA on Somatic Nuclear Transfer of Guangxi Luchuan-pig |
| EndPage | 2375 |
| ExternalDocumentID | xnnyxb201106073 40584721 |
| GrantInformation_xml | – fundername: 国家自然科学基金资助项目; 农业部"转基因生物新品种培育重大专项项目; 广西自然科学基金重点项目; 广西大学创新团队建设计划项目 funderid: (30860039); (2009ZX08010-023B); (2010GXNSFD013021); (2010) |
| GroupedDBID | -04 .3O 2B. 2B~ 2RA 5XA 5XE 92G 92I 92L ABDBF ACGFS ALMA_UNASSIGNED_HOLDINGS CCEZO CHDYS CQIGP CW9 C~G DYU EOJEC ESX OBODZ OZF TCJ TGD U1G U5N W95 ~WA 4A8 93N PSX |
| ID | FETCH-LOGICAL-c583-1506e86e9a79a5a16e856fc68419d3bfc2e383c097c83d2806cbb94779f2b6643 |
| ISSN | 1001-4829 |
| IngestDate | Wed Nov 06 04:37:31 EST 2024 Wed Feb 14 10:32:12 EST 2024 |
| IsPeerReviewed | false |
| IsScholarly | true |
| Issue | 6 |
| Keywords | 体细胞核移植 猪 TSA 卵母细胞 5-aza-dc |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c583-1506e86e9a79a5a16e856fc68419d3bfc2e383c097c83d2806cbb94779f2b6643 |
| Notes | QIN Xiao-e,WANG Meng,SONG Xue-mei,LU Sheng-sheng,LU Ke-huan（State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Animal Reproduction Institute,Guangxi University,Guangxi Nanning 530004,China） 51-1213/S To investigate the effect of 5-aza-20-deoxycytidine（5-aza-dC） and trichostatin A（TSA）on the somatic nuclear transfer of Guangxi Luchuan-pig,5-aza-dC and TSA were used to treat somatic cells and cloned embryos for decreasing the level of DNA methylation.The result showed that,the development rate of morular was significantly increased compared to control group（P0.05）with the treatment of donor cells,cloned embryos and continuous treatment of both donor cells and cloned embryos with a combination of 0.01 μM 5-aza-dC and 0.05 μM TSA,whereas the rate of blastocyte was not significantly different among the groups.It was concluded that： the development of cloned porcine embryos could be enhanced by the treatment of both donor cells and cloned embryos with a combination of 5-aza-dC and |
| PageCount | 4 |
| ParticipantIDs | wanfang_journals_xnnyxb201106073 chongqing_primary_40584721 |
| PublicationCentury | 2000 |
| PublicationDate | 2011 |
| PublicationDateYYYYMMDD | 2011-01-01 |
| PublicationDate_xml | – year: 2011 text: 2011 |
| PublicationDecade | 2010 |
| PublicationTitle | 西南农业学报 |
| PublicationTitleAlternate | Southwest China Journal of Agricultural Sciences |
| PublicationTitle_FL | Southwest China Journal of Agricultural Sciences |
| PublicationYear | 2011 |
| Publisher | 亚热带农业生物资源保护与利用国家重点实验室/广西大学动物繁殖研究所,广西南宁,530004 |
| Publisher_xml | – name: 亚热带农业生物资源保护与利用国家重点实验室/广西大学动物繁殖研究所,广西南宁,530004 |
| SSID | ssib002263352 ssib051375617 ssj0001933376 ssib001101446 ssib036440650 |
| Score | 1.8642904 |
| Snippet | ... S828; 为探讨甲基转移酶抑制剂5-aza-dc、去乙酰基转移酶抑制剂TSA对广西陆川猪体细胞核移植效率的影响,用5-aza-dc、TSA处理供体细胞和重构胚.结果显示,5... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 2372 |
| SubjectTerms | 5-aza-dc TSA 体细胞核移植 卵母细胞 猪 |
| Title | 5-aza-dc和TSA对广西陆川猪体细胞核移植的影响 |
| URI | http://lib.cqvip.com/qk/94396X/201106/40584721.html https://d.wanfangdata.com.cn/periodical/xnnyxb201106073 |
| Volume | 24 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LaxRBEB5iBPEiioqJD3JIHyfuTD-mGvHQsw-CoBc3EE_LvDY5baImEHNVckou5qSgeFAEL-JJQwj5M242_gureno3Y1h8wTJ0d1VXf1U1dFfN9vR43iznQRrVuqmvi6zmC5EWvs6CxO_mIs0DvEMgpUTx_gM1vyDuLcrFicm7lV1L62vpXLY59r2S__EqtqFf6S3Zf_DsSCg2YBn9i1f0MF7_ysfSTzYTP89YUzIdMqi3HxoqmxaLNRXwGrdYE5iRtqCZ1gyUJUVMN1gzwl7MYC_B4gbTnFri2PIAA850kzUVMzUWA5GMpVILxqC2uzYMhBXYYHFgkXAkVYPeCgBJvXRkC4rpuh0XSAjBbjCjSDgY5B_eCm5YU6KuMbBiDJxigSaD2Omj4xMKspYU1D22miJ4tJWssiAobEMKmke3rFrxUEo-fMY7un0taGNBR2QjxE3QwGE8rRgKE04qaGY0mSOW1mrKyqlZ5pb1R6m8cN1RKRIItpeVg3qTiWMyd6lbrEg3iJw_IHS-RxI5T5POaJOyBSWHrXF3hiSSiSpukBYJVGBH1AsCa0EUrqxnaoSKCtoiUcRM-0nr40cZuZ-8gqtoXXIK-CtrI-2-E-AeULnFMxSVSeKXlZCXn2RyURVW5bgVm2ul7YpNQ8yNhnBn69LBuvwkUhntH93o9Z5tpDZ2Vchxxjsb4jpDO3objxZOsgH6DnX19LxQ0RuGwzrHVIASlGFdBohxmO3bR7Sac24_TznCdc6bdaBv_w4yHdSyvNJbeozhp30bsNdNekuVwLV90bvgMs4ZU04fl7yJzeXL3p3h1NF_uY2TRv_zXn_v8PjD4Y9XW_2vbwbbn74f7A72t46fvz16923wcf_o_c7g9Yv-wZf-7s4Vr91qtuvzvvuOip9J4D6dIVqAKnQS6UQmAVak6mYKRKBznnazsODAs5qOMuA57bTI0lSLKNLdMFWYsVz1JnsrveKaNyME6CBLVChBiqjIEi61yLkKoJtDLsIpb3qkd2e1PC6ngykhhsBhMOXNOEN03Bz6tHPKkdN_ZrnunS__1qHfDW9y7cl6cRPzgrX0lvX-Twr3v-Y |
| link.rule.ids | 315,783,787,4031,27935,27936,27937 |
| linkProvider | CAB International |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=5-aza-dc%E5%92%8CTSA%E5%AF%B9%E5%B9%BF%E8%A5%BF%E9%99%86%E5%B7%9D%E7%8C%AA%E4%BD%93%E7%BB%86%E8%83%9E%E6%A0%B8%E7%A7%BB%E6%A4%8D%E7%9A%84%E5%BD%B1%E5%93%8D&rft.jtitle=%E8%A5%BF%E5%8D%97%E5%86%9C%E4%B8%9A%E5%AD%A6%E6%8A%A5&rft.au=%E7%A7%A6%E5%B0%8F%E5%A8%A5&rft.au=%E7%8E%8B%E7%8C%9B&rft.au=%E5%AE%8B%E9%9B%AA%E6%A2%85&rft.au=%E5%8D%A2%E6%99%9F%E7%9B%9B&rft.date=2011&rft.pub=%E4%BA%9A%E7%83%AD%E5%B8%A6%E5%86%9C%E4%B8%9A%E7%94%9F%E7%89%A9%E8%B5%84%E6%BA%90%E4%BF%9D%E6%8A%A4%E4%B8%8E%E5%88%A9%E7%94%A8%E5%9B%BD%E5%AE%B6%E9%87%8D%E7%82%B9%E5%AE%9E%E9%AA%8C%E5%AE%A4%2F%E5%B9%BF%E8%A5%BF%E5%A4%A7%E5%AD%A6%E5%8A%A8%E7%89%A9%E7%B9%81%E6%AE%96%E7%A0%94%E7%A9%B6%E6%89%80%2C%E5%B9%BF%E8%A5%BF%E5%8D%97%E5%AE%81%2C530004&rft.issn=1001-4829&rft.volume=24&rft.issue=6&rft.spage=2372&rft.epage=2375&rft_id=info:doi/10.3969%2Fj.issn.1001-4829.2011.06.073&rft.externalDocID=xnnyxb201106073 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F94396X%2F94396X.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fxnnyxb%2Fxnnyxb.jpg |