5-aza-dc和TSA对广西陆川猪体细胞核移植的影响

为探讨甲基转移酶抑制剂5-aza-dc、去乙酰基转移酶抑制剂TSA对广西陆川猪体细胞核移植效率的影响,用5-aza-dc、TSA处理供体细胞和重构胚。结果显示,5-Aza-dc联合TSA处理供体细胞、重构胚以及连续处理供体细胞和重构胚,桑椹胚率均显著高于对照组（P〈0.05）,但各组间囊胚率差异不显著。说明5-aza-dc联合TSA处理供体细胞和克隆胚能提高猪克隆胚的发育能力,尽管差异不显著。...

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Bibliographic Details
Published in西南农业学报 Vol. 24; no. 6; pp. 2372 - 2375
Main Author 秦小娥 王猛 宋雪梅 卢晟盛 卢克焕
Format Journal Article
LanguageChinese
Published 亚热带农业生物资源保护与利用国家重点实验室/广西大学动物繁殖研究所,广西南宁,530004 2011
Subjects
5-aza-dc
TSA
体细胞核移植
卵母细胞
猪
体细胞核移植
猪
TSA
卵母细胞
5-aza-dc
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Summary:为探讨甲基转移酶抑制剂5-aza-dc、去乙酰基转移酶抑制剂TSA对广西陆川猪体细胞核移植效率的影响,用5-aza-dc、TSA处理供体细胞和重构胚。结果显示,5-Aza-dc联合TSA处理供体细胞、重构胚以及连续处理供体细胞和重构胚,桑椹胚率均显著高于对照组（P〈0.05）,但各组间囊胚率差异不显著。说明5-aza-dc联合TSA处理供体细胞和克隆胚能提高猪克隆胚的发育能力,尽管差异不显著。
Bibliography:QIN Xiao-e,WANG Meng,SONG Xue-mei,LU Sheng-sheng,LU Ke-huan（State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Animal Reproduction Institute,Guangxi University,Guangxi Nanning 530004,China）
51-1213/S
To investigate the effect of 5-aza-20-deoxycytidine（5-aza-dC） and trichostatin A（TSA）on the somatic nuclear transfer of Guangxi Luchuan-pig,5-aza-dC and TSA were used to treat somatic cells and cloned embryos for decreasing the level of DNA methylation.The result showed that,the development rate of morular was significantly increased compared to control group（P0.05）with the treatment of donor cells,cloned embryos and continuous treatment of both donor cells and cloned embryos with a combination of 0.01 μM 5-aza-dC and 0.05 μM TSA,whereas the rate of blastocyte was not significantly different among the groups.It was concluded that： the development of cloned porcine embryos could be enhanced by the treatment of both donor cells and cloned embryos with a combination of 5-aza-dC and
ISSN:1001-4829
DOI:10.3969/j.issn.1001-4829.2011.06.073