Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica

• Published in: Yàowu shi͡p︡in fenxi Vol. 22; no. 3; pp. 296 - 302
• Main Authors: Do, Quy Diem, Angkawijaya, Artik Elisa, Tran-Nguyen, Phuong Lan, Huynh, Lien Huong, Soetaredjo, Felycia Edi, Ismadji, Suryadi, Ju, Yi-Hsu
• Format: Journal Article
• Language: English
• Published: China (Republic : 1949- ) Elsevier B.V 01.09.2014; Food and Drug Administration; Taiwan Food and Drug Administration
• Subjects: 2,2-diphenyl-1-picrylhydrazyl; Acetone; Aluminum; antioxidant activity; Antioxidants; Antioxidants activity; Chloride; drugs; Effect of solvent; Equivalence; Ethanol; Flavonoids; Food analysis; freeze drying; Gallic acid; Herbal medicine; in vitro studies; Limnophila; Limnophila aromatica; Medicinal plants; methanol; Methods; Molecular weight; Original; Oxidative stress; Phenolic compounds; Phenols; Polyphenols; Potassium; Quercetin; Sodium; Solvents; South East Asia; spices; Total phenol content; United States > US; Southeast Asia; Germany; South East Asia; Limnophila aromatica; Total phenol content; Antioxidants activity; Food analysis; Effect of solvent
• ISSN: 1021-9498; 2224-6614
• DOI: 10.1016/j.jfda.2013.11.001

Limnophila aromatica is commonly used as a spice and a medicinal herb in Southeast Asia. In this study, water and various concentrations (50%, 75%, and 100%) of methanol, ethanol, and acetone in water were used as solvent in the extraction of L. aromatica. The antioxidant activity, total phenolic content, and total flavonoid content of the freeze-dried L. aromatica extracts were investigated using various in vitro assays. The extract obtained by 100% ethanol showed the highest total antioxidant activity, reducing power and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity. The same extract also exhibited the highest phenolic content (40.5 mg gallic acid equivalent/g of defatted L. aromatica) and the highest flavonoid content (31.11 mg quercetin equivalent/g of defatted L. aromatica). The highest extraction yield was obtained by using 50% aqueous acetone. These results indicate that L. aromatica can be used in dietary applications with a potential to reduce oxidative stress.