An Archaeal Immune System Can Detect Multiple Protospacer Adjacent Motifs (PAMs) to Target Invader DNA

• Published in: The Journal of biological chemistry Vol. 287; no. 40; pp. 33351 - 33363
• Main Authors: Fischer, Susan, Maier, Lisa-Katharina, Stoll, Britta, Brendel, Jutta, Fischer, Eike, Pfeiffer, Friedhelm, Dyall-Smith, Mike, Marchfelder, Anita
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 28.09.2012; American Society for Biochemistry and Molecular Biology
• Subjects: Amino Acid Motifs; Amino Acid Sequence; Archaea; Archaea - metabolism; Base Sequence; BASIC BIOLOGICAL SCIENCES; Biochemistry & Molecular Biology; Computational Biology - methods; CRISPR/Cas; DNA - chemistry; Haloferax - immunology; Haloferax - metabolism; Haloferax volcanii; Microbiology; Models, Genetic; Molecular Sequence Data; Mutation; Nucleic Acids - chemistry; PAM; Plasmids - metabolism; Pyrococcus - metabolism; RNA; RNA - metabolism; RNA Processing; Sequence Homology, Amino Acid; Species Specificity; Sulfolobus - immunology; Sulfolobus - metabolism; Microbiology; RNA; Haloferax volcanii; Archaea; RNA Metabolism; RNA Processing; CRISPR/Cas; PAM

The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system provides adaptive and heritable immunity against foreign genetic elements in most archaea and many bacteria. Although this system is widespread and diverse with many subtypes, only a few species have...