A novel poly (glycine) biosensor towards the detection of indigo carmine: A voltammetric study

• Published in: Yàowu shi͡p︡in fenxi Vol. 26; no. 1; pp. 292 - 299
• Main Author: Manjunatha, Jamballi Gangadharappa Gowda
• Format: Journal Article
• Language: English
• Published: China (Republic : 1949- ) Elsevier B.V 01.01.2018; Food and Drug Administration; Taiwan Food and Drug Administration
• Subjects: antifouling activities; Antifouling substances; Biosensors; buffers; Carbon; carbon electrodes; Catalysis; Catalytic activity; Cyclic voltammetry; detection limit; Electrochemical analysis; Electrochemistry; Electrodes; Experiments; Glycine; Indigo; Indigo carmine; Original; Oxidation; pH effects; phosphates; Poly (glycine) modified paste carbon electrode; Polymerization; Reaction mechanisms; Response time; Voltammetry; Mumbai India; India; Cyclic voltammetry; Indigo carmine; Poly (glycine) modified paste carbon electrode
• ISSN: 1021-9498; 2224-6614
• DOI: 10.1016/j.jfda.2017.05.002

The electrochemical behavior of indigo carmine (IC) at poly (glycine) modified carbon paste electrode (PGMCPE) was investigated by cyclic and differential pulse voltammetry. The oxidation peak of IC was observed in phosphate buffer of pH 6.5. The influence of different pH, scan rate, and concentration were analyzed. The probable reaction mechanism involved in the oxidation of IC was also proposed. Results showed that PGMCPE a remarkable electrocatalytic activity for the oxidation of IC under optimal conditions. The electrocatalytic response of the sensor was proportional to the IC concentration in the range of (2 × 10−6–1 × 10−5 M) and (1.5 × 10−5–6 × 10−5 M) with a limit of detection 11 × 10−8 M and limit of quantification 3.6 × 10−7 M. The modified electrode demonstrated many advantages such as simple preparation, high sensitivity, low detection of limit, excellent catalytic activity, short response time, and remarkable antifouling property toward IC and its oxidation product. In this work, carbon paste electrode modified with glycine by electropolymerization method. The fabricated electrode was used for the electrochemical sensor applications. Poly (glycine) modified carbon paste electrode shows excellent sensitivity, wide range and good detection limit towards electrochemical determination of indigo carmine. [Display omitted] •Highly sensitive detection of indigo carmine by cyclic voltammetry.•A high-performance poly (glycine) modified carbon paste electrode.•Great applicability towards clinical samples.•A wide dynamic range, detection limit and remarkably high sensitivity are obtained.