Antioxidant activity, neuroprotective properties and bioactive constituents analysis of varying polarity extracts from Eucalyptus globulus leaves

• Published in: Yàowu shi͡p︡in fenxi Vol. 26; no. 4; pp. 1293 - 1302
• Main Authors: González-Burgos, Elena, Liaudanskas, Mindaugas, Viškelis, Jonas, Žvikas, Vaidotas, Janulis, Valdimaras, Gómez-Serranillos, M. Pilar
• Format: Journal Article
• Language: English
• Published: China (Republic : 1949- ) Elsevier B.V 01.10.2018; Food and Drug Administration; Taiwan Food and Drug Administration
• Subjects: Accuracy; Acetone; Alzheimer's disease; antioxidant activity; Antioxidants; Bioactive constituents; Biological activity; cell viability; Chlorogenic acid; Constituents; Dietary supplements; Ethanol; Eucalyptus; Eucalyptus globulus; Gas flow; Herbal tea; Hydrogen peroxide; In vitro methods and tests; leaf extracts; Leaves; Lipid peroxidation; Methanol; Neuroprotection; neuroprotective effect; Original; Oxidative stress; Peroxidation; Phenolic compounds; Phenols; Polarity; Properties (attributes); Raw materials; Tea; United Kingdom > UK; United States > US; Germany; Antioxidants; Neuroprotection; Eucalyptus globulus; Bioactive constituents
• ISSN: 1021-9498; 2224-6614
• DOI: 10.1016/j.jfda.2018.05.010

Eucalyptus globulus is employed as herbal tea and therapeutical purposes. In this work, it is investigated for first time the neuroprotective activities, based on antioxidant properties, of varying polarity extracts (acetone, ethanol and methanol) from E. globulus leaves and elucidate their main bioactive constituents. Methanol and acetone extracts contained the highest phenolic compounds amount and chlorogenic acid was the major compound identified by UPLC-ESI-MS/MS. Moreover, the three tested extracts showed significant antioxidant properties, varying their potency depending on the in vitro technique used. Furthermore, E.globulus extracts were effective in ameliorating H2O2-induced oxidative stress by increasing cell viability, GSH levels and antioxidant enzymes activity and, by decreasing ROS production and lipid peroxidation levels in SH-SY5Y cells. Taken together, E.globulus leaves extracts could be used as raw material for food and pharmaceutical supplements for their high content in antioxidant compounds with health benefits properties against oxidative stress. [Display omitted] •Elucidation of varying polarity extracts from Eucalyptus globulus leaves.•The major identified compound was chlorogenic acid.•E. globulus extracts had potent in vitro antioxidant activities.•E. globulus extracts prevented H2O2-induced oxidative stress in SH-SY5Y cells.