Notch signalling mediates reproductive constraint in the adult worker honeybee

• Published in: Nature communications Vol. 7; no. 1; p. 12427
• Main Authors: Duncan, Elizabeth J., Hyink, Otto, Dearden, Peter K.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 03.08.2016; Nature Publishing Group; Nature Portfolio
• Subjects: 13; 13/1; 14/19; 38/77; 38/91; 631/181/2469; 631/181/2806; 631/601/1466; 631/80/86; Aging - metabolism; Animals; Bees; Bees - drug effects; Bees - genetics; Bees - metabolism; Eggs; Evolution; Female; Females; Gene Expression Regulation - drug effects; Hierarchy, Social; Humanities and Social Sciences; Insect Proteins - metabolism; Insects; Ligands; multidisciplinary; Ovaries; Ovary - drug effects; Ovary - metabolism; Pheromones; Pheromones - pharmacology; Physiology; Receptors, Notch - metabolism; Reproduction - drug effects; Reproduction - genetics; Roles; Science; Science (multidisciplinary); Signal Transduction - drug effects
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/ncomms12427

The hallmark of eusociality is the reproductive division of labour, in which one female caste reproduces, while reproduction is constrained in the subordinate caste. In adult worker honeybees ( Apis mellifera ) reproductive constraint is conditional: in the absence of the queen and brood, adult worker honeybees activate their ovaries and lay haploid male eggs. Here, we demonstrate that chemical inhibition of Notch signalling can overcome the repressive effect of queen pheromone and promote ovary activity in adult worker honeybees. We show that Notch signalling acts on the earliest stages of oogenesis and that the removal of the queen corresponds with a loss of Notch protein in the germarium. We conclude that the ancient and pleiotropic Notch signalling pathway has been co-opted into constraining reproduction in worker honeybees and we provide the first molecular mechanism directly linking ovary activity in adult worker bees with the presence of the queen. In honeybees, pheromones produced by the queen inhibit reproduction by workers and enforce a eusocial division of labour. Here, Duncan, Hyink and Dearden show that this inhibition is mediated by the Notch signalling pathway in the workers' ovaries.