Identification and mapping of a new powdery mildew resistance gene on chromosome 6D of common wheat

• Published in: Theoretical and applied genetics Vol. 123; no. 7; pp. 1099 - 1106
• Main Authors: Ma, Hongqi, Kong, Zhongxin, Fu, Bisheng, Li, Na, Zhang, Lixia, Jia, Haiyan, Ma, Zhengqiang
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.11.2011; Springer; Springer Nature B.V
• Subjects: Agricultural research; Agriculture; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Blumeria graminis; Blumeria graminis f. sp. tritici; chromosome mapping; Chromosome Mapping - methods; Chromosomes; Chromosomes, Plant - genetics; Classical genetics, quantitative genetics, hybrids; Cultivars; Disease; Diseases and pests; evolution; Fundamental and applied biological sciences. Psychology; Gene expression; Genes; Genes, Plant; Genetic aspects; genetic distance; Genetic Linkage; genetic markers; Genetic Markers - genetics; Genetic research; Genetic Techniques; Genetics of eukaryotes. Biological and molecular evolution; Genomics; Germplasm; Health aspects; Life Sciences; loci; Mildew; Models, Genetic; Original Paper; Pathogens; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Diseases - immunology; Plant Genetics and Genomics; Plant immunology; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Powders; powdery mildew; Pteridophyta, spermatophyta; Triticum - genetics; Triticum aestivum; Vegetals; Virulence; Wheat; China; Powdery Mildew Resistance; Common Wheat; Wheat Breeding Program; Simple Sequence Repeat Marker; Powdery Mildew; Monocotyledones; Gene; Gramineae; Angiospermae; Chromosome; Genetics; Spermatophyta; Agriculture; Identification; Fungus resistance; Triticum aestivum
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-011-1651-3

Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most serious wheat diseases. The rapid evolution of the pathogen’s virulence, due to the heavy use of resistance genes, necessitates the expansion of resistance gene diversity. The common wheat line D57 is highly resistant to powdery mildew. A genetic analysis using an F2 population derived from the cross of D57 with the susceptible cultivar Yangmai 158 and the derived F2:3 lines indicated that D57 carries two dominant powdery mildew resistance genes. Based on mapping information of polymorphic markers identified by bulk segregant analysis, these two genes were assigned to chromosomes 5DS and 6DS. Using the F2:3 lines that segregated in a single-gene mode, closely linked PCR-based markers were identified for both genes, and their chromosome assignments were confirmed through linkage mapping. The gene on chromosome 5DS was flanked by Xgwm205 and Xmag6176, with a genetic distance of 8.3 cM and 2.8 cM, respectively. This gene was 3.3 cM from a locus mapped by the STS marker MAG6137, converted from the RFLP marker BCD1871, which was 3.5 cM from Pm2. An evaluation with 15 pathogen isolates indicated that this gene and Pm2 were similar in their resistance spectra. The gene on chromosome 6DS was flanked by co-segregating Xcfd80 and Xmag6139 on one side and Xmag6140 on the other, with a genetic distance of 0.7 cM and 2.7 cM, respectively. This is the first powdery mildew resistance gene identified on chromosome 6DS, and plants that carried this gene were highly resistant to all of the 15 tested pathogen isolates. This gene was designated Pm45. The new resistance gene in D57 could easily be transferred to elite cultivars due to its common wheat origin and the availability of closely linked molecular markers.