Lactobacillus pentosus GMNL-77 inhibits skin lesions in imiquimod-induced psoriasis-like mice

• Published in: Yàowu shi͡p︡in fenxi Vol. 25; no. 3; pp. 559 - 566
• Main Authors: Chen, Yi-Hsing, Wu, Chieh-Shan, Chao, Ya-Husan, Lin, Chi-Chen, Tsai, Hui-Yun, Li, Yi-Rong, Chen, Yi-Zhen, Tsai, Wan-Hua, Chen, Yu-Kuo
• Format: Journal Article
• Language: English
• Published: China (Republic : 1949- ) Elsevier B.V 01.07.2017; Food and Drug Administration; Taiwan Food and Drug Administration
• Subjects: Aminoquinolines; Animals; Antigens; Antiviral drugs; Autoimmune diseases; Bacteria; CD4 antigen; CD4-positive T-lymphocytes; Cytokines; Dermatitis; drugs; Hyperplasia; Imiquimod; In vivo methods and tests; Inflammation; Interleukin 17; Interleukin 22; Interleukin 23; Keratinocytes; Laboratory animals; Lactobacillus; Lactobacillus pentosus; Lactobacillus pentosus GMNL-77; Lesions; Lymphocytes; Lymphocytes T; messenger RNA; Mice; mRNA; Oral administration; Original; Polymerase chain reaction; Probiotics; Psoriasis; quantitative polymerase chain reaction; Rodents; Scaling; Skin; Skin diseases; skin lesions; Spleen; Studies; T cells; tumor necrosis factor-alpha; Tumor necrosis factor-TNF; Tumor necrosis factor-α; United Kingdom > UK; United States > US; cytokines; T cells; inflammation; psoriasis; imiquimod; Lactobacillus pentosus GMNL-77
• ISSN: 1021-9498; 2224-6614
• DOI: 10.1016/j.jfda.2016.06.003

Psoriasis, which is regarded as a T-cell-mediated chronic inflammatory skin disease, is characterized by hyperproliferation and poor differentiation of epidermal keratinocytes. In this study, we aimed to determine the in vivo effect of a potentially probiotic strain, Lactobacillus pentosus GMNL-77, in imiquimod-induced epidermal hyperplasia and psoriasis-like skin inflammation in BALB/c mice. Oral administration of L. pentosus GMNL-77 significantly decreased erythematous scaling lesions. Real-time polymerase chain reaction showed that treatment with L. pentosus GMNL-77 significantly decreased the mRNA levels of proinflammatory cytokines, including tumor necrosis factor-alpha, interleukin (IL)-6, and the IL-23/IL-17A axis-associated cytokines (IL-23, IL-17A/F, and IL-22) in the skin of imiquimod-treated mice. In addition, we found that L. pentosus GMNL-77 decreased the spleen weights of the imiquimod-treated group and reduced the numbers of IL-17- and IL-22-producing CD4+ T cells in the spleen. In conclusion, the present study provides insight into the potential use of L. pentosus GMNL-77 in the future treatment of psoriasis. [Display omitted]