Liquid chromatography-tandem mass spectrometry method for simultaneous determination of albendazole and albendazole sulfoxide in human plasma for bioequivalence studies

• Published in: Journal of pharmaceutical analysis Vol. 6; no. 4; pp. 226 - 234
• Main Authors: Rathod, Dhiraj M., Patel, Keyur R., Mistri, Hiren N., Jangid, Arvind G., Shrivastav, Pranav S., Sanyal, Mallika
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.08.2016; Department of Chemistry, Kadi Sarv%Bioanalytical Research Department, Accutest Research Lab, Bodakdev, Ahmedabad 380059, India%Department of Chemistry, School of Sciences, Gujarat University, Ahmedabad 380009, India%Department of Chemistry, St. Xavier's Col ege, Navrangpura, Ahmedabad 380009, India; Xi'an Jiaotong University; Elsevier
• Subjects: Albendazole; Albendazole sulfoxide; Bioequivalence study; LC > MS/MS; Original; Solid phase extraction; 丙硫咪唑; 亚砜; 人血浆; 同时测定; 液相色谱-串联质谱法; 生物等效性; 阿苯达唑; 高效液相色谱-串联质谱; Solid phase extraction; Bioequivalence study; Albendazole; LC > MS/MS; Albendazole sulfoxide; LC-MS/MS
• ISSN: 2095-1779; 2214-0883
• DOI: 10.1016/j.jpha.2016.02.002

An improved high performance liquid chromatography-tandem mass spectrometry（LC-MS/MS） method has been developed for sensitive and rapid determination of albendazole（ABZ） and its active metabolite,albendazole sulfoxide（ABZSO）,in the positive ionization mode.The method utilized solid phase extraction（SPE） for sample preparation of the analytes and their deuterated internal standards（ISs） from100 μL human plasma.The chromatography was carried out on Hypurity C_（18） column using acetonitrile-2.0 mM ammonium acetate,pH 5.0（80：20,v/v） as the mobile phase.The assay exhibited a linear response over the concentration range of 0.200-50.0 ng/mL for ABZ and 3.00-600 ng/mL for ABZSO.The recoveries of the analytes and ISs ranged from 86.03%-89.66%and 89.85%-98.94%,respectively.Matrix effect,expressed as IS-normalized matrix factors,ranged from 0.985 to 1.042 for the both analytes.The method was successfully applied for two separate studies in healthy subjects using single dose of 400 mg conventional tablets and 400 mg chewable ABZ tablets,respectively.