Further Studies on the Structure of Polysaccharides from the Bark of Melia azadirachta

• Published in: Chemical & pharmaceutical bulletin Vol. 32; no. 4; pp. 1385 - 1391
• Main Authors: FUJIWARA, TADAMI, SUGISHITA, ETSUKO, TAKEDA, TADAHIRO, OGIHARA, YUKIO, SHIMIZU, MASAKI, NOMURA, TAKEO, TOMITA, YUTAKA
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 01.01.1984; Maruzen; Japan Science and Technology Agency
• Subjects: Biological and medical sciences; C-NMR; General pharmacology; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Molecular structure; Dicotyledones; Angiospermae; Antiinflammatory agent; Polyoside; Isolation; Spermatophyta; Meliaceae; Biological activity; Medicinal plant
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.32.1385

Water-soluble polysaccharides, designated as GIIa and GIIIa, were isolated together with GIa and GIb from the bark of Melia azadirachta (Meliaceae), and their anti-inflammatory effect on carrageenan-induced edema was tested. GIIa and GIIIa each gave a single peak on high-performance liquid chromatography and gel filtration. Methylation, periodate oxidation and carbon-13 nuclear magnetic resonance spectroscopic studies suggested that GIIa is composed of the following repeating unit : α-D-G1c1→4α-D-G1c1→3α-D-G1c1→3α-D-G1c6←1α-L-ArafGIIIa is [numbrical formula] a branched arabinofucoglucan containing a main chain of 1→4-linked α-D-glucopyranosyl units substituted in the 6 position with side chains of α-L-arabinofuranose and β-L-fucopyranose.