Proteomics study of human cord blood reticulocyte-derived exosomes

• Published in: Scientific reports Vol. 8; no. 1; pp. 14046 - 11
• Main Authors: Díaz-Varela, Míriam, de Menezes-Neto, Armando, Perez-Zsolt, Daniel, Gámez-Valero, Ana, Seguí-Barber, Joan, Izquierdo-Useros, Nuria, Martinez-Picado, Javier, Fernández-Becerra, Carmen, del Portillo, Hernando A.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 19.09.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 101/28; 101/58; 13; 13/1; 13/31; 14/19; 631/337/475; 631/80; 692/53; 82/16; 82/29; Blood Banks; Cell culture; Cell Culture Techniques; Cells, Cultured; Cord blood; Cèl·lules dendrítiques; Dendritic cells; Dendritic Cells - cytology; Dendritic Cells - metabolism; Erythrocytes; Erythropoiesis; Exosome Biogenesis; Exosomes; Exosomes - metabolism; Extracellular Vesicles; Fetal Blood - cytology; Fetal Blood - metabolism; Human Reticulocyte-derived; Humanities and Social Sciences; Humans; Immunoelectron microscopy; Mass Spectrometry; Mass spectroscopy; Microscopy, Immunoelectron; multidisciplinary; Nanotechnology; Over-represention GO Analysis; Proteins; Proteomics; Proteomics - methods; Proteïnes; Receptors, Transferrin - metabolism; Reticulocytes; Reticulocytes - cytology; Reticulocytes - metabolism; Science; Science (multidisciplinary); Transferrin Receptor; Transferrins; Over-represention GO Analysis; Transferrin Receptor; Human Reticulocyte-derived; Extracellular Vesicles; Exosome Biogenesis
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-32386-2

Reticulocyte-derived exosomes ( Rex ), extracellular vesicles of endocytic origin, were initially discovered as a cargo-disposal mechanism of obsolete proteins in the maturation of reticulocytes into erythrocytes. In this work, we present the first mass spectrometry-based proteomics of human Rex ( HuRex ). HuRex were isolated from cultures of human reticulocyte-enriched cord blood using different culture conditions and exosome isolation methods. The newly described proteome consists of 367 proteins, most of them related to exosomes as revealed by gene ontology over-representation analysis and include multiple transporters as well as proteins involved in exosome biogenesis and erythrocytic disorders. Immunoelectron microscopy validated the presence of the transferrin receptor. Moreover, functional assays demonstrated active capture of HuRex by mature dendritic cells. As only seven proteins have been previously associated with HuRex , this resource will facilitate studies on the role of human reticulocyte-derived exosomes in normal and pathological conditions affecting erythropoiesis.