TAS1 trans-Acting siRNA Targets Are Differentially Regulated at Low Temperature, and TAS1 trans-Acting siRNA Mediates Temperature-Controlled At1g51670 Expression

• Published in: Bioscience, biotechnology, and biochemistry Vol. 74; no. 7; pp. 1435 - 1440
• Main Authors: KUME, Kohei, TSUTSUMI, Ken-ichi, SAITOH, Yasushi
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 2010; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Arabidopsis; Arabidopsis - genetics; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Base Sequence; Biochemistry; Biological and medical sciences; Biotechnology; Cleavage; Control; Durability; Enzymes; Fluctuation; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Plant - genetics; microRNA (miRNA); noncoding RNA; Plants (organisms); RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Small Interfering - genetics; RNAi; robustness; Temperature; trans-acting siRNA (tasiRNA); Temperature; RNAi; RNA interference; noncoding RNA; Targeting; RNA; microRNA (miRNA); Micro RNA; robustness; siRNA; Gene silencing; Target; Regulation(control); trans-acting siRNA (tasiRNA); Low temperature
• ISSN: 0916-8451; 1347-6947; 1347-6947
• DOI: 10.1271/bbb.100111

To endure considerable fluctuations in temperature, plants need precise regulation of temperature-controlled gene expression. In this study, the involvement of TAS1 trans-acting siRNA (tasiRNA) in temperature-controlled gene expression was examined in Arabidopsis. The accumulation of TAS1 tasiRNA was downregulated at 4 °C. Concomitant with the reduction of TAS1 tasiRNA-mediated cleavage, expression of At1g51670, a target of TAS1 tasiRNA, was upregulated at 4 °C in the wild type but not in a dicer-like enzyme (DCL) 4 mutant (dcl4-2), which is impaired in tasiRNA biogenesis. The expression of At4g29760 and of At5g18040, further TAS1 tasiRNA targets, was upregulated both in the wild type and in dcl4-2 at 4 °C. However, after shifting the temperature to 22 °C, low-temperature-induced expression of At4g29760 rapidly dropped in the wild type, but not in dcl4-2. Thus TAS1 tasiRNA acted as a sweeper for the clearance of excess amounts of At4g29760 transcripts. Our data suggest that differential regulation of TAS1 tasiRNA targets is involved in temperature-controlled gene expression.