Nucleotide sequences of glycoprotein I and E genes of equine herpesvirus type 4

• Published in: Journal of Veterinary Medical Science Vol. 60; no. 2; pp. 219 - 225
• Main Authors: Damiani, A.M. (Tokyo Univ. (Japan). Faculty of Agriculture), Matsumura, T, Yokoyama, N, Maeda, K, Miyazawa, T, Kai, C, Mikami, T
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.02.1998; Japan Science and Technology Agency
• Subjects: Amino Acid Sequence; Animals; Base Sequence; CABALLOS; Cells, Cultured; CHEVAL; EQUINE HERPESVIRUS; equine herpesvirus type 4; GENE; GENES; Genome, Viral; GLICOPROTEINAS; glycoprotein E; glycoprotein I; GLYCOPROTEINE; GLYCOPROTEINS; HERPESVIRUS EQUIN; HERPESVIRUS EQUINO; HORSES; Kidney; Molecular Sequence Data; NUCLEOTIDE SEQUENCE; Open Reading Frames; Restriction Mapping; SECUENCIA NUCLEOTIDICA; Sequence Alignment; Sequence Homology, Amino Acid; SEQUENCE NUCLEOTIDIQUE; Varicellovirus - genetics; Varicellovirus - physiology; Viral Envelope Proteins - biosynthesis; Viral Envelope Proteins - chemistry; Viral Envelope Proteins - genetics
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.60.219

The nucleotide sequences of the glycoprotein I (gI) and E (gE) genes of equine herpesvirus type 4 (EHV-4) strain TH20 were determined. The predicted region encoding the EHV-4 gI gene is 1,263 nucleotides, corresponding to a polypeptide of 420 amino acids in length. The predicted region encoding the EHV-4 gE gene is 1,647 nucleotides, corresponding to a polypeptide of 548 amino aids in length. The EHV-4 gI and gE genes show 74% and 86% identity at the amino acid level with those of equine herpesvirus type 1 (EHV-1), respectively. Furthermore, we have found an open reading frame homologous to the EHV-1 gene 75, which overlaps in part with the 3' end of EHV-4 gE gene. These sequence data will be useful for development of a modified live vaccine against equine herpesvirus type 1 and 4 infections