Microbiome analysis in women with endometriosis: Does a microbiome exist in peritoneal fluid and ovarian cystic fluid?

• Published in: Reproductive medicine and biology Vol. 21; no. 1; pp. e12441 - n/a
• Main Authors: Oishi, Sugiko, Mekaru, Keiko, Tanaka, Suguru E., Arai, Wataru, Ashikawa, Kyota, Sakuraba, Yoshiyuki, Nishioka, Mikiko, Nakamura, Rie, Miyagi, Maho, Akamine, Kozue, Aoki, Yoichi
• Format: Journal Article
• Language: English
• Published: Japan John Wiley & Sons, Inc 01.01.2022; John Wiley and Sons Inc; Wiley
• Subjects: 16S rRNA; Abdomen; Bacteria; Dysbacteriosis; Endometriosis; Endometrium; Genital tract; Laparoscopy; microbiome; Microbiomes; Microorganisms; Multivariate analysis; Original; ovarian cystic fluid; Ovaries; Patients; Peritoneal fluid; Peritoneum; rRNA 16S; Secretions; Streptococcus infections; Surgery; Tumors; Vagina; Womens health; United States > US; Germany; endometriosis; 16S rRNA; peritoneal fluid; microbiome; ovarian cystic fluid
• ISSN: 1445-5781; 1447-0578
• DOI: 10.1002/rmb2.12441

Purpose To investigate the relationship between the microbiome of the female genital tract and endometriosis. Methods This prospective cohort study included 36 women who underwent laparoscopic surgery for ovarian tumor from July 2019 to April 2020. Of them, 18 had endometriosis, and 18 did not have endometriosis. Vaginal secretions, endometrial fluid, peritoneal fluid, and ovarian cystic fluid were collected during surgery. Next‐generation sequencing of bacterial 16S rRNA was performed to characterize the microbiome. Results Specific microbiomes were not detected in either peritoneal fluid or ovarian cystic fluid regardless of the presence or absence of endometriosis and the type of cyst. When the cutoff value of infectious bacterial abundance in the vagina was set as 64.3%, there were many cases more than a cutoff value in the endometriosis group significantly (p = 0.01). When the cutoff value of infectious bacterial abundance in the endometrium was set as 18.6%, there were many cases more than a cutoff level in the endometriosis cases significantly (p = 0.02). Conclusion Peritoneal fluid and ovarian cystic fluid are almost sterile, although dysbiosis may occur in the vaginal and endometrial microbiome in women with endometriosis.