副猪嗜血杆菌血清5型分离株生长曲线与致病力研究

本文对副猪嗜血杆菌血清5型生长曲线进行了研究。结果表明:种子液接种后6 h开始进入对数生长期,培养24 h后活菌数达到最高峰,20~30 h活菌数维持在同一个水平,进入稳定期,30 h后活菌数呈现下降趋势,进入衰退期。该曲线提供了副猪嗜血杆菌生长规律,为疫苗研制提供了理论基础。...

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Published in西南农业学报 Vol. 24; no. 5; pp. 1972 - 1976
Main Author 王大鹏 吴家强 于江 丛晓燕 杜以军 孙文博 张玉玉 时建立 李俊 周顺 王金宝
Format Journal Article
LanguageChinese
Published 山东省畜禽疫病防治与繁育重点实验室,山东济南250100%山东省畜禽疫病防治与繁育重点实验室,山东济南250100 2011
山东省农业科学院畜牧兽医研究所,山东济南250100%青岛农业大学动物科技学院,山东青岛,266109%青岛农业大学动物科技学院,山东青岛266109
青岛农业大学动物科技学院,山东青岛266109
山东省畜禽疫病防治与繁育重点实验室,山东济南250100
山东省农业科学院畜牧兽医研究所,山东济南250100
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Summary:本文对副猪嗜血杆菌血清5型生长曲线进行了研究。结果表明:种子液接种后6 h开始进入对数生长期,培养24 h后活菌数达到最高峰,20~30 h活菌数维持在同一个水平,进入稳定期,30 h后活菌数呈现下降趋势,进入衰退期。该曲线提供了副猪嗜血杆菌生长规律,为疫苗研制提供了理论基础。
Bibliography:WANG Da-peng,WU Jia-qiang,YU Jiang,CONG Xiao-yan,DU Yi-jun,SUN Wen-bo,ZHANG Yu-yu,SHI Jian-li,LI Jun,ZHOU Shun,WANG Jin-bao(1.College of Animal Science and Technology,Qingdao Agricultural University,Shandong Qingdao 266109, China;2.Shandong Key Laboratory of Animal Disease Control and Breeding,Shandong Jinan 250100,China;3. Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Shandong Jinan 250100,China)
In this paper,the growth curve of Haemophilus parasuis serotype 5 strain was studied.The result showed that the growth of Haemophilus parasuis serotype 5 strain went in the logarithmic growth phase after 6 hours post-inoculation(hpi), and reached the peak after 24 h,and then remained at the same level afer 20-30 h,which indicated that the bacteria entered the stable phase.After 30 h, the bacteria showed a downward trend,meaning going into the recession phase.The establishment of the growth curve supplied a better understanding of the growth characteristics of Haemophilu
ISSN:1001-4829
DOI:10.3969/j.issn.1001-4829.2011.05.072