过表达miR-30b对人胃癌细胞株SGC-7901和AGS生物学功能及其肿瘤形成的影响

目的研究过表达miR-30b对人胃癌细胞株SGC-7901和AGS的增殖、细胞周期、凋亡、侵袭等生物学功能的影响及对体内肿瘤形成的抑制作用。方法SGC-7901和AGS细胞中转染miR-30b类似物和miR-对照后，qRT-PCR检测转染后细胞miR-30b的表达变化；Westernblot检测eIF5A2蛋白的表达；CCK-8检测SGC-7901和AGS细胞增殖；流式细胞术检测细胞周期和凋亡；Transwell实验检测细胞体外侵袭能力。转染miR-30b类似物和miR-对照的SGC-7901和AGS细胞，分别注射裸鼠体内，观察肿瘤的形成及肿瘤组织中eIF5A2蛋白的表达。结果qRT-PCR结...

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Bibliographic Details
Published in天津医药 Vol. 45; no. 7; pp. 677 - 681
Main Author 陈翠翠;梁焕坤;李康燕;程承武;刘细潘;黎杰星;钟树海;张立成;李来庆
Format Journal Article
LanguageChinese
Published 广州优迪生物科技股份有限公司 510663%解放军第88医院肿瘤科 2017
Subjects
微RNAs;细胞增殖;细胞周期;细胞凋亡;肿瘤侵润;miR-30b
细胞周期
细胞凋亡
microRNAs
miR-30b
cell proliferation
cell cycle
neoplasm invasiveness
apoptosis
肿瘤侵润
微RNAs
细胞增殖
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Summary:目的研究过表达miR-30b对人胃癌细胞株SGC-7901和AGS的增殖、细胞周期、凋亡、侵袭等生物学功能的影响及对体内肿瘤形成的抑制作用。方法SGC-7901和AGS细胞中转染miR-30b类似物和miR-对照后，qRT-PCR检测转染后细胞miR-30b的表达变化；Westernblot检测eIF5A2蛋白的表达；CCK-8检测SGC-7901和AGS细胞增殖；流式细胞术检测细胞周期和凋亡；Transwell实验检测细胞体外侵袭能力。转染miR-30b类似物和miR-对照的SGC-7901和AGS细胞，分别注射裸鼠体内，观察肿瘤的形成及肿瘤组织中eIF5A2蛋白的表达。结果qRT-PCR结果表明miR-30b类似物组的相对表达量显著高于miR-对照组（P＜0.05）；Westernblot结果显示，miR-30b类似物组的eIF5A2蛋白表达降低；CCK-8实验表明miR-30b类似物组中细胞增殖受到抑制；流式细胞术显示miR-30b类似物组的细胞周期减慢，凋亡增加；在Transwell迁移实验中，miR-30b类似物组细胞侵袭能力明显低于miR-对照组（P＜0.05）。细胞体内成瘤实验显示，miR-30b过表达抑制肿瘤的形成，肿瘤组织中eIF5A2蛋白表达降低。结论miR-30b过表达抑制人胃癌细胞的增殖、侵袭和肿瘤的形成，降低eIF5A2蛋白的表达，为胃癌治疗提供了一个潜在的靶点。
Bibliography:Objective To investigate the effect of overexpression of miR-30b on the proliferation,cell cycle,apoptosis and invasion of gastric cancer cell line SGC-7901and AGS,and the inhibitory effect on the tumor formation in vivo.Methods SGC-7901and AGS cells were transfected with miR-30b mimics and miR-control,and qRT-PCR was used to detect the expression levels of miR-30b.Western blot assay was used to detect the expression of eIF5A2protein.CCK-8assay was used to measure the cell proliferation.Flow cytometry was used to analyze cell cycle and apoptosis.Transwell assay was used to detect cell invasion.In addition,the SGC-7901and AGS cells transfected with miR-30b mimics and miRcontrol were injected into nude mice to observe the tumor formation and the expression of eIF5A2protein in vivo.Results Results of qRT-PCR showed that the relative expression of miR-30b was significantly higher than that of miR-control group(P<0.05).Western blot assay showed that the expression of eIF5A2protein was decreased in miR-30b mimic
ISSN:0253-9896
DOI:10.11958/20170352