棉籽粕固态发酵过程及其动力学模型构建

为了研究枯草芽孢杆菌Bs-1在工业化条件下发酵棉籽粕的过程,该文采用槽式发酵方式,测定了发酵过程中物料堆温、含水率、粗蛋白、酸溶性蛋白和游离棉酚质量分数;并采用Logistic方程建立芽孢杆菌Bs-1生长模型,在其与粗蛋白、酸溶性蛋白和游离棉酚质量分数相关性分析的基础上,构建了固态发酵动力学模型并进行了发酵成本分析。结果表明：棉籽粕槽式发酵过程中的温度和含水率均呈现一定的变化规律;粗蛋白质量分数、酸溶性蛋白质量分数分别较发酵前提高了9.28%和46.51%,游离棉酚质量分数降低了42.31%。Bs-1的生长与粗蛋白质量分数（R2=0.831）和酸溶性蛋白质量分数（R2=0.867）呈正相关,与...

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Bibliographic Details
Published in农业工程学报 Vol. 28; no. 13; pp. 199 - 204
Main Author 吴逸飞 孙宏 姚晓红 王新 汤江武
Format Journal Article
LanguageChinese
Published 浙江省农业科学院植物保护与微生物研究所,杭州,310021 2012
Subjects
动力学
发酵
棉籽粕
模型
游离棉酚
粗蛋白
酸溶性蛋白
动力学
酸溶性蛋白
游离棉酚
模型
棉籽粕
粗蛋白
发酵
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ISSN1002-6819
DOI10.3969/j.issn.1002-6819.2012.13.032

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Summary:为了研究枯草芽孢杆菌Bs-1在工业化条件下发酵棉籽粕的过程,该文采用槽式发酵方式,测定了发酵过程中物料堆温、含水率、粗蛋白、酸溶性蛋白和游离棉酚质量分数;并采用Logistic方程建立芽孢杆菌Bs-1生长模型,在其与粗蛋白、酸溶性蛋白和游离棉酚质量分数相关性分析的基础上,构建了固态发酵动力学模型并进行了发酵成本分析。结果表明：棉籽粕槽式发酵过程中的温度和含水率均呈现一定的变化规律;粗蛋白质量分数、酸溶性蛋白质量分数分别较发酵前提高了9.28%和46.51%,游离棉酚质量分数降低了42.31%。Bs-1的生长与粗蛋白质量分数（R2=0.831）和酸溶性蛋白质量分数（R2=0.867）呈正相关,与游离棉酚质量分数（R2=0.976）呈负相关;建立了棉籽粕固态发酵动力学模型。分析表明,该模型计算值与试验值能较好吻合,且发酵成本低。表明该模型可应用于工业化生产,对实现发酵棉籽粕的产业化生产,具有较好的参考价值。
Bibliography:11-2047/S
In order to investigate the changes of the concentrations of free gossypol（FG）,crude protein（CP） and acid soluble protein（ASP） in the solid-state fermentation of cottonseed meal by Bacillus subtilis Bs-1,the temperatures,moisture contents,as well as the concentrations of CP,ASP and FG were measured in windrow-type aerobic fermentation.A growth model of Bs-1 was established using the equation of logistic.The kinetic models of cottonseed meal fermentation were built by accessing the correlation between the growth of B.subtilis Bs-1 and the contents of CP,ASP,and FG.Results showed that both temperature and moisture had regular changing trends in the process of fermentation.The concentrations of CP and ASP increased by 9.28% and 46.51%,respectively,whereas the FG content dropped by 42.31% after fermentation.Correlations between Bs-1 number and CP（R2=0.831）/ASP（R2=0.867） were positive.The Bs-1 population was negatively correlated with FG content（R2=0.976）.The predicted values of the models agreed well wit
ISSN:1002-6819
DOI:10.3969/j.issn.1002-6819.2012.13.032