Isolation and Identification of Avian Nephropathogenic Infectious Bronchitis Virus

In November 2009, a respiratory disease with rapid transmission, rapid onset and mortality of about 8% appeared many times in a large chicken farm in Jiangsu Province of China. Necropsy revealed tracheal bleeding, kidney enlargement and white-spotted kidney. An isolate from the farm was identified a...

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Published inAnimal husbandry and feed science Vol. 2; no. 5; pp. 26 - 28
Main Authors Gao, W, Bao, Y-Q, Zhang, J-J, Gu, C-G
Format Journal Article
LanguageEnglish
Published 01.05.2010
Subjects
Avian infectious bronchitis virus
Infectious bronchitis virus
PCR扩增
分离
呼吸系统疾病
大型养鸡场
肾型IBV
肾型鸡传染性支气管炎病毒
血凝试验
鉴定
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Summary:In November 2009, a respiratory disease with rapid transmission, rapid onset and mortality of about 8% appeared many times in a large chicken farm in Jiangsu Province of China. Necropsy revealed tracheal bleeding, kidney enlargement and white-spotted kidney. An isolate from the farm was identified as an avian infectious bronchitis virus （IBV） by chicken embryo inoculation, hemagglutination assay, virus interference assay, animal regression and tracheal rings culture. The complete ,S1 gene was amplified by RT-PCR, and its homology to that of the vaccine strains com- monly used in China was analyzed with DNAStar software. Therefore, the IBV isolate was initially classified into nephropathogenic IBV and named IBV JS09 strain.
Bibliography:Nephropathogenic infectious bronchitis virus; Isolation; Chickens
In November 2009, a respiratory disease with rapid transmission, rapid onset and mortality of about 8% appeared many times in a large chicken farm in Jiangsu Province of China. Necropsy revealed tracheal bleeding, kidney enlargement and white-spotted kidney. An isolate from the farm was identified as an avian infectious bronchitis virus （IBV） by chicken embryo inoculation, hemagglutination assay, virus interference assay, animal regression and tracheal rings culture. The complete ,S1 gene was amplified by RT-PCR, and its homology to that of the vaccine strains com- monly used in China was analyzed with DNAStar software. Therefore, the IBV isolate was initially classified into nephropathogenic IBV and named IBV JS09 strain.
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ISSN:1943-9911