加工番茄马铃薯Y病毒一步法RT—PCR检测及CP基因的克隆与序列分析

本文通过一步法RT-PCR技术，检测新疆加工番茄马铃薯Y病毒（Potato virus Y，PVY）感染率。结果表明，新疆加工番茄马铃薯Y病毒感染率为25％。选取10个样品利用RT-PCR技术，克隆CP基因，获得807bp全长CP基因，编码268个氨基酸。CP基因序列比对分析与系统进化树分析表明，侵染新疆加工番茄的马铃薯Y病毒属于PVY^N：O株系。...

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Bibliographic Details
Published in西南农业学报 Vol. 26; no. 6; pp. 2340 - 2344
Main Author 梁学超 向本春 程朝玲 苏海娣 郑银英
Format Journal Article
LanguageChinese
Published 石河子大学生命科学学院,石河子大学农业生物技术重点实验室,新疆石河子832003 2013
Subjects
CP基因
一步法RT—PCR
加工番茄
马铃薯Y病毒
CP gene
加工番茄
Processing tomato
Potato virus Y
CP基因
马铃薯Y病毒
One-step RT-PCR
一步法RT-PCR
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ISSN1001-4829

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Summary:本文通过一步法RT-PCR技术，检测新疆加工番茄马铃薯Y病毒（Potato virus Y，PVY）感染率。结果表明，新疆加工番茄马铃薯Y病毒感染率为25％。选取10个样品利用RT-PCR技术，克隆CP基因，获得807bp全长CP基因，编码268个氨基酸。CP基因序列比对分析与系统进化树分析表明，侵染新疆加工番茄的马铃薯Y病毒属于PVY^N：O株系。
Bibliography:Potato virus Y; Processing tomato; One-step RT-PCR; CP gene
The study analyzes potato virus Y prevalence of infection in processing tomato in Xinjiang by the one-step reverse transcription-pol- ymerase chain reaction（ RT-PCR）, the result showed that 25 % processing tomato could be infected with PVY. The CP gene included 807bp, and could encode a protein with 268 amino acids. CP gene of PVY strain were analyzed with informatics tools, and the molecular variation characters among different PVY strains were proved up. According to these results, PVY which infected processing tomato in Xinjiang was identified as an isolate of PVY^N：O strain.
51-1213/S
LIANG Xue-chao, XINAG Ben-chun, CHENG Chao-ling, SHU Hai-di, ZHENG Yin-ying （College of Life Sciences, Key Laboratory of Agriculture Biotechnology of Shihezi University, Shihezi University, Xinjiang Uygur Autono- mous Region, Xinjiang Shihezi 832003 ,China）
ISSN:1001-4829