ISSR分子标记对杧果实生苗父本的早期鉴定

[目的]采用ISSR分子标记技术对杧果实生苗进行亲本早期鉴定,为提高杧果育种效率提供参考.[方法]采用ISSR分子标记技术,筛选不同引物鉴定97-1实生苗的父本.[结果]采用优化的ISSR反应体系,从18条引物中筛选获得4条特异引物UBC-848、UBC-857、UBC-864和UBC-868,其扩增条带多、稳定性强、多态性高、背景清晰.利用引物UBC-848、UBC-857、UBC-864、UBC-868分别对四川红柁、紫花柁和97-1实生苗进行扩增,获得的扩增片段长度在100～2000bp,四川红杧与97-1实生苗、紫花杧与97-1实生苗的共有特异性条带约为180和380 bp、280和3...

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Bibliographic Details
Published in南方农业学报 Vol. 45; no. 1; pp. 7 - 11
Main Author 张宇 王长江 唐志鹏 周导军
Format Journal Article
LanguageChinese
Published 广西大学农学院,南宁530005 2014
广西亚热带作物研究所,南宁530001
广西作物遗传改良生物技术重点开放实验室,南宁530007%邹城市林业局林业站,山东邹城,273500%广西大学农学院,南宁,530005
Subjects
97-1实生苗
ISSR标记
亲本早期鉴定
四川红杧
杧果
紫花杧
97-1实生苗
early parental analysis
ISSR标记
Sichuan red-skin mango
97-1 seedling
紫花杧
Mangifera indica L
杧果
Jyhua mango
ISSR marker
四川红杧
亲本早期鉴定
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Summary:[目的]采用ISSR分子标记技术对杧果实生苗进行亲本早期鉴定,为提高杧果育种效率提供参考.[方法]采用ISSR分子标记技术,筛选不同引物鉴定97-1实生苗的父本.[结果]采用优化的ISSR反应体系,从18条引物中筛选获得4条特异引物UBC-848、UBC-857、UBC-864和UBC-868,其扩增条带多、稳定性强、多态性高、背景清晰.利用引物UBC-848、UBC-857、UBC-864、UBC-868分别对四川红柁、紫花柁和97-1实生苗进行扩增,获得的扩增片段长度在100～2000bp,四川红杧与97-1实生苗、紫花杧与97-1实生苗的共有特异性条带约为180和380 bp、280和380 bp、180和680 bp、680和770bp.[结论]采用ISSR分子标记技术和引物UBC-848、UBC-857、UBC-864、UBC-868构建了杧果实生苗亲本鉴定体系,可鉴定出97-1实生苗的父本为四川红杧,97-1实生苗是紫花杧和四川红杧的杂交种.
Bibliography:45-1381/S
[Objective]Early identification and selection of parents of mango seedlings by ISSR molecular markers were conducted to provide references for improving mango breeding efficiency based on ISSR molecular markers. [Method]Using ISSR marker technology, different primers were screened to identify male parent of 97-1 seedling. [ Result]According to the optimized ISSR reaction system, four specific primers were chosen from 18 primers（UBC-848, UBC-857,UBC-864 and UBC-868）, which had many amplified bands, strong stability, high polymorphism and clear backgrounds. Jyhna mango, Sichuan red-skin mango and 97-1 seedlings were amplified using primers UBC-846,UBC-857,UBC-864 and UBC-868. The length of the amplified fragments was 100-2000 bp. The size of specific bands of Sichuan red-skin mango, Jyhua mango and 97-1 seedlings was 180 and 380 bp, 280 and 380 bp, 180 and 680 bp, and 680 and 770 bp. [Conclusion]Through the identification system construction of mango seedling parents using ISSR molecular markers and pr
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2014.1.7