AKT-targeted anti-inflammatory activity of Panax ginseng calyx ethanolic extract

• Published in: Journal of ginseng research Vol. 42; no. 4; pp. 496 - 503
• Main Authors: Han, Sang Yun, Kim, Juewon, Kim, Eunji, Kim, Su Hwan, Seo, Dae Bang, Kim, Jong-Hoon, Shin, Song Seok, Cho, Jae Youl
• Format: Journal Article
• Language: English
• Published: Korea (South) Elsevier B.V 01.10.2018; 고려인삼학회; Elsevier
• Subjects: AKT; anti-inflammatory activity; calyx of berry; nuclear factor-κB; Panax ginseng; 기타의약학; nuclear factor-κB; AKT; calyx of berry; Panax ginseng; anti-inflammatory activity; nuclear factor-kB
• ISSN: 1226-8453; 2093-4947
• DOI: 10.1016/j.jgr.2017.06.003

Korean ginseng (Panax ginseng) plays an anti-inflammatory role in a variety of inflammatory diseases such as gastritis, hepatitis, and colitis. However, inflammation-regulatory activity of the calyx of the P. ginseng berry has not been thoroughly evaluated. To understand whether the calyx portion of the P. ginseng berry is able to ameliorate inflammatory processes, an ethanolic extract of P. ginseng berry calyx (Pg-C-EE) was prepared, and lipopolysaccharide-activated macrophages and HEK293 cells transfected with inflammation-regulatory proteins were used to test the anti-inflammatory action of Pg-C-EE. The ginsenoside contents of Pg-C-EE were analyzed by HPLC. Suppressive activity of Pg-C-EE on NO production, inflammatory gene expression, transcriptional activation, and inflammation signaling events were examined using the Griess assay, reverse transcription-polymerization chain reaction, luciferase activity reporter gene assay, and immunoblotting analysis. Pg-C-EE reduced NO production and diminished mRNA expression of inflammatory genes such as cyclooxygenase-2, inducible NO synthase, and tumor necrosis factor-α in a dose-dependent manner. This extract suppressed luciferase activity induced only by nuclear factor-κB. Interestingly, immunoblotting analysis results demonstrated that Pg-C-EE reduced the activities of protein kinase B (AKT)1 and AKT2. These results suggest that Pg-C-EE may have nuclear-factor-κB-targeted anti-inflammatory properties through suppression of AKT. The calyx of the P. ginseng berry is an underused part of the ginseng plant, and development of calyx-derived extracts may be useful for treatment of inflammatory diseases.