Accelerated bioethanol fermentation by using a novel yeast immobilization technique: Microtube array membrane

• Published in: Process biochemistry (1991) Vol. 50; no. 10; pp. 1509 - 1515
• Main Authors: Chen, Chien-Chung, Wu, Chien-Hui, Wu, Jhih-Jhong, Chiu, Chien-Chih, Wong, Chien-Hsuan, Tsai, Min-Lang, Lin, Hong-Ting Victor
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.10.2015
• Subjects: adverse effects; Arrays; batch fermentation; Bioethanol; catalytic activity; encapsulation; ethanol fermentation; ethanol production; Fermentation; Glucose; Immobilization; immobilized cells; inoculum; Kluyveromyces marxianus; Membranes; Microtube array membrane (MTAM); Polylactic acid; porosity; Productivity; Repeated-batch; Yeast; yeasts; Repeated-batch; Kluyveromyces marxianus; Bioethanol; Microtube array membrane (MTAM); Fermentation
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2015.06.006

•A novel cell immobilization technique for bioethanol production was introduced.•Microtube array membrane was stable in 15% ethanol during shaking cultivation.•MTAM-encapsulated yeasts showed better bioethanol productivity. Cell immobilization is a way to isolate or localize intact cells in a certain space and maintain their catalytic activity. Immobilized cells can effectively reduce the negative effects of inhibitors and the processing cost of inoculum preparation for continuous or fed-batch fermentation of microorganisms. In this study, a novel yeast immobilization technique using renewable poly-l-lactic acid (PLLA) microtube array membrane (MTAM) was thoroughly evaluated for bioethanol fermentation. PLLA-MTAM was shown to be stable in 15% (v/v) ethanol solution during shaking cultivation. A yeast encapsulation efficiency of 67–70% was obtained, and the yeasts in MTAMs with greater porosity showed greater bioethanol productivity. The MTAM-immobilized Kluyveromyces marxianus, prepared using in situ and siphon methods, were evaluated using 5% (w/v) glucose fermentation. Improved glucose consumption and bioethanol production were observed in batch bioethanol fermentation. In 7 cycles during repeated-batch fermentation, the immobilized yeasts prepared using the in situ method showed a maximum CEtOH of 24.23g/L, maximum YP/S of 0.48g/g, and r PEtOH of 2.69g/L h. Our data indicated that the PLLA-MTAM immobilized yeasts significantly enhanced bioethanol productivity and was a novel, promising technology for bioethanol fermentation.