MicroRNA-124 inhibits cancer cell growth through PTB1/PKM1/PKM2 feedback cascade in colorectal cancer

• Published in: Cancer letters Vol. 363; no. 1; pp. 17 - 27
• Main Authors: Taniguchi, Kohei, Sugito, Nobuhiko, Kumazaki, Minami, Shinohara, Haruka, Yamada, Nami, Nakagawa, Yoshihito, Ito, Yuko, Otsuki, Yoshinori, Uno, Bunji, Uchiyama, Kazuhisa, Akao, Yukihiro
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 10.07.2015; Elsevier Limited
• Subjects: 3' Untranslated Regions; Angiogenesis; Animals; Apoptosis; Autophagy; Carrier Proteins - genetics; Carrier Proteins - metabolism; Case-Control Studies; Cell growth; Cell Line, Tumor; Colorectal cancer; Colorectal Neoplasms - enzymology; Colorectal Neoplasms - genetics; Colorectal Neoplasms - pathology; Colorectal Neoplasms - therapy; Energy Metabolism; Feedback, Physiological; Female; Free radicals; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Neoplastic; Genes; Genetic Therapy - methods; Hematology, Oncology and Palliative Medicine; Heterogeneous-Nuclear Ribonucleoproteins - genetics; Heterogeneous-Nuclear Ribonucleoproteins - metabolism; Humans; Kinases; Male; Membrane Proteins - genetics; Membrane Proteins - metabolism; Metabolism; Mice, Inbred BALB C; Mice, Nude; MicroRNAs - administration & dosage; MicroRNAs - genetics; MicroRNAs - metabolism; miR-124; Oxidative Stress; Patients; PKM; Polypyrimidine Tract-Binding Protein - genetics; Polypyrimidine Tract-Binding Protein - metabolism; Proteins; PTB1; RNA Interference; Studies; Thyroid Hormone-Binding Proteins; Thyroid Hormones - genetics; Thyroid Hormones - metabolism; Time Factors; Transfection; Tumor Burden; Tumors; Vascular endothelial growth factor; Warburg effect; Xenograft Model Antitumor Assays; Japan; DDX6; ORF; heterogeneous nuclear ribonucleoprotein; Colorectal cancer; mRNA; open reading frame; miR-124; colorectal adenoma; messenger RNA; PTB1; reactive oxygen species; programmed cell death; polymerase chain reaction; Warburg effect; 3′ untranslated region; pyruvate kinase muscle; hnRNP; polypyrimidine tract-binding protein 1; DEAD (Asp-Glu-Ala-Asp) box helicase 6; 3′UTR; siRNA; PCD; PKM; CRA; CRC; miR; ROS; short-interfering RNA; microRNA; PCR
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/j.canlet.2015.03.026

Highlights • The expression levels of miR-124 were decreased in clinical colorectal cancer (CRC) and adenoma (CRA) patients' samples. • MiR-124 induced apoptosis and/or autophagic survival in human colon cancer cells. • MiR-124 targeted PTB1 to modulate the Warburg effect through the switching of PKM isoform expression from PKM2 to PKM1.