Protective effect of Pasteurella multocida cell-free antigen and toxoid against challenge with toxigenic strains of Pasteurella multocida in mice

• Published in: Journal of Veterinary Medical Science Vol. 65; no. 6; pp. 737 - 740
• Main Authors: Uchida, C. (Kyoto Biken Lab., Uji (Japan)), Kimura, Y, Kubota, S, Sasaki, O
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.06.2003; Japan Science and Technology Agency
• Subjects: Animals; ANTIGENS; Antigens, Bacterial - immunology; Bacterial Vaccines - immunology; cell-free antigen; MICE; Pasteurella Infections - prevention & control; PASTEURELLA MULTOCIDA; Pasteurella multocida - immunology; toxin; TOXINS; Toxoids - immunology; Vaccination
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.65.737

The cell-free antigen (CFA) obtained from the culture supernatant of Pasteurella multocida (P. multocida) and the toxin (PMT) purified from CFA were inactivated and mixed with oil adjuvant to prepare a trial vaccine. Both of the mice immunized with CFA and PMT toxoid vaccine were noticeably protected against intratracheal challenge with toxigenic strains of P. multocida. Nevertheless, the protective indices of the mice immunized with CFA vaccine indicate that it is more protective and clears away the bacteria more promptly than in the mice immunized with PMT vaccine. The results suggested that CFA would possibly be good as an effective antigen to toxigenic strains of P. multocida infection.