Safety and Tumor Specificity of Cetuximab-IRDye800 for Surgical Navigation in Head and Neck Cancer

• Published in: Clinical cancer research Vol. 21; no. 16; pp. 3658 - 3666
• Main Authors: Rosenthal, Eben L, Warram, Jason M, de Boer, Esther, Chung, Thomas K, Korb, Melissa L, Brandwein-Gensler, Margie, Strong, Theresa V, Schmalbach, Cecelia E, Morlandt, Anthony B, Agarwal, Garima, Hartman, Yolanda E, Carroll, William R, Richman, Joshua S, Clemons, Lisa K, Nabell, Lisle M, Zinn, Kurt R
• Format: Journal Article
• Language: English
• Published: United States 15.08.2015
• Subjects: Adult; Aged; Aged, 80 and over; Antibodies, Monoclonal, Humanized - administration & dosage; Carcinoma, Squamous Cell - diagnostic imaging; Carcinoma, Squamous Cell - pathology; Carcinoma, Squamous Cell - surgery; Cetuximab - administration & dosage; Cetuximab - adverse effects; ErbB Receptors; Female; Fluorescent Dyes - administration & dosage; Fluorescent Dyes - adverse effects; Head and Neck Neoplasms - diagnostic imaging; Head and Neck Neoplasms - pathology; Head and Neck Neoplasms - surgery; Humans; Indoles - administration & dosage; Indoles - adverse effects; Indoles - pharmacokinetics; Male; Middle Aged; Optical Imaging; Radiography; Squamous Cell Carcinoma of Head and Neck
• ISSN: 1078-0432; 1557-3265
• DOI: 10.1158/1078-0432.ccr-14-3284

Positive margins dominate clinical outcomes after surgical resections in most solid cancer types, including head and neck squamous cell carcinoma. Unfortunately, surgeons remove cancer in the same manner they have for a century with complete dependence on subjective tissue changes to identify cancer in the operating room. To effect change, we hypothesize that EGFR can be targeted for safe and specific real-time localization of cancer. A dose escalation study of cetuximab conjugated to IRDye800 was performed in patients (n = 12) undergoing surgical resection of squamous cell carcinoma arising in the head and neck. Safety and pharmacokinetic data were obtained out to 30 days after infusion. Multi-instrument fluorescence imaging was performed in the operating room and in surgical pathology. There were no grade 2 or higher adverse events attributable to cetuximab-IRDye800. Fluorescence imaging with an intraoperative, wide-field device successfully differentiated tumor from normal tissue during resection with an average tumor-to-background ratio of 5.2 in the highest dose range. Optical imaging identified opportunity for more precise identification of tumor during the surgical procedure and during the pathologic analysis of tissues ex vivo. Fluorescence levels positively correlated with EGFR levels. We demonstrate for the first time that commercially available antibodies can be fluorescently labeled and safely administered to humans to identify cancer with sub-millimeter resolution, which has the potential to improve outcomes in clinical oncology.