Antimicrobial resistance of commensal Escherichia coli from food-producing animals in Russia

• Published in: Veterinary World Vol. 13; no. 10; pp. 2053 - 2061
• Main Authors: Makarov, Dmitry A, Ivanova, Olga E, Karabanov, Sergey Yu, Gergel, Maria A, Pomazkova, Anastasia V
• Format: Journal Article
• Language: English
• Published: India Veterinary World 01.10.2020
• Subjects: Ampicillin; Analysis; Animal husbandry; Animals; Antimicrobial agents; Antimicrobial resistance; Biological products; Biomaterials; Breakpoints; Cattle; Cefotaxime; Ciprofloxacin; Colistin; commensal bacteria; critically important antimicrobials; Drug resistance; E coli; Escherichia coli; Farms; Food; Genetic markers; Information management; Microbial drug resistance; multidrug resistance; Poultry; Public health; Swine; Tetracyclines; China; Russia; multidrug resistance; animals; commensal bacteria; antimicrobial resistance; critically important antimicrobials; Escherichia coli
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2020.2053-2061

Commensal is an important indicator of antimicrobial resistance (AMR) in animals and food of animal origin. Therefore, it was recommended by the World Health Organization and OIE for inclusion in resistance surveillance programs. At the same time, the data on isolates from animals in Russia are scarce. The aim of this work was to determine the current prevalence of resistance and genetic markers of non-pathogenic commensal collected from major food-producing animals (poultry, pigs, and cows) in different regions of Russia and to compare these data with data from other countries to prioritize antimicrobials for limiting their use according to the National Action Plan. Samples (n=306) were collected from biomaterial of chicken, turkey, cows, and pigs raised on 11 farms in the European part of Russia, Siberia, and North Caucasus. Isolates (n=306) of were tested for resistance to 11 antimicrobials from ten classes using the broth microdilution method. MICs were interpreted against EUCAST microbiological and clinical breakpoints. For data analysis and statistical processing, the AMRcloud online platform was used. The data are presented in comparison with other countries. In Russia, higher levels of microbiological and clinical resistance of to critically important antimicrobials, including colistin, cefotaxime, and ciprofloxacin, were found compared to other countries, especially in poultry: About 30% of isolates from chicken were resistant to colistin, 8% to cefotaxime, and 88% to ciprofloxacin according to EUCAST ECOFFs. Only 10% of isolates from cows were resistant to cefotaxime. About 47% of isolates of from chicken had a moderate relative resistance for ampicillin and 56% for tetracycline. For most antimicrobials, isolates from cows demonstrated a lower resistance than isolates from poultry and pigs. All tested isolates from chicken, turkey, and pigs showed a simultaneous microbiological resistance to at least three classes of antimicrobials. No pan-resistant isolates were found. High levels of AMR of commensal from poultry, especially for critically important drugs, are a matter of concern and should be taken into account when choosing antimicrobials to be restricted for use in animal husbandry according to the National Action Plan.