Improved hydrophilic interaction chromatography method for the identification and quantification of glucosinolates

An improved hydrophilic interaction liquid chromatography (HILIC) method has been developed to separate members of a closely related family of chemoprotective phytochemicals called glucosinolates. This method exploits the emergence of a second generation of HILIC chemistry, using a silica-based perm...

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Bibliographic Details
Published inJournal of Chromatography A Vol. 1154; no. 1; pp. 469 - 472
Main Authors Wade, Kristina L., Garrard, Ian J., Fahey, Jed W.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 22.06.2007
Elsevier
Subjects
Biological and medical sciences
Broccoli
Carcinogenesis, carcinogens and anticarcinogens
Chemical agents
Chemoprotection
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
food analysis
food composition
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Glucoiberin
Glucoraphanin
glucosinolates
Glucosinolates - analysis
HPLC
hydrophilic interaction liquid chromatography
Imidoesters - analysis
liquid chromatography
Medical sciences
Phytochemical
Sulforaphane glucosinolate
Tumors
Broccoli
Chemoprotection
Sulforaphane glucosinolate
Glucoraphanin
Glucoiberin
Phytochemical
HPLC
Chemical analysis
Glucosinolate
Vegetables
Hydrophilic Interaction Liquid chromatography
HPLC chromatography
Anticarcinogen
Ultraviolet detector
Organic isothiocyanate
Qualitative analysis
Quantitative analysis
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Summary:An improved hydrophilic interaction liquid chromatography (HILIC) method has been developed to separate members of a closely related family of chemoprotective phytochemicals called glucosinolates. This method exploits the emergence of a second generation of HILIC chemistry, using a silica-based permanently zwitterionic stationary phase. These columns are more robust, durable, and glucosinolates separations are more reproducible than with the original polyhydroxyethyl aspartamide columns. Furthermore, the HILIC system that we report herein permits much greater alteration of the mobile phase composition for customized separation of glucosinolates from plant extracts, across a wide spectrum of polarity.
Bibliography:http://dx.doi.org/10.1016/j.chroma.2007.04.034
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ISSN:0021-9673
1873-3778
DOI:10.1016/j.chroma.2007.04.034