Metabarcoding Analysis of Bacterial Communities Associated with Media Grow Bed Zones in an Aquaponic System

The development of environmentally sustainable plant and fish production in aquaponic systems requires a complete understanding of the systems’ biological components. In order to better understand the role of microorganisms in this association, we studied the bacterial communities in the dry, root,...

Full description

Saved in:
Bibliographic Details
Published inInternational journal of microbiology Vol. 2020; no. 2020; pp. 1 - 10
Main Authors Kasozi, Nasser, Wilhelmi, Brendan, Kaiser, Horst
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Publishing Corporation 01.10.2020
Hindawi
John Wiley & Sons, Inc
Hindawi Limited
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:The development of environmentally sustainable plant and fish production in aquaponic systems requires a complete understanding of the systems’ biological components. In order to better understand the role of microorganisms in this association, we studied the bacterial communities in the dry, root, and mineralized zones of a flood-and-drain media bed aquaponic system. Bacterial communities were characterized using metabarcoding of the V3-V4 16S rRNA regions obtained from paired-end Illumina MiSeq reads. Proteobacteria, Actinobacteria, and Bacteroidetes accounted for more than 90% of the total community in the dry zone and the effluent water. These phyla also accounted for more than 68% of the total community in the root and mineralized zones. The genera Massilia, Mucilaginibacter, Mizugakiibacter, and Rhodoluna were most dominant in the dry, root, and mineralized zones and in the effluent water, respectively. The number of shared operational taxonomic units (OTUs) for the three zones was 241, representing 7.15% of the total observed OTUs. The number of unique OTUs in samples from dry zone, root zone, mineralized zone, and effluent water was 485, 638, 445, and 383, respectively. The samples from the root zone harbored more diverse communities than either the dry or mineralized zones. This study is the first to report on the bacterial community within the zones of a flood-and-drain media bed. Thus, this information will potentially accelerate studies on other microbial communities involved in the bioconversion of nitrogen compounds and mineralization within these types of aquaponic systems.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Academic Editor: Shady Amin
ISSN:1687-918X
1687-9198
DOI:10.1155/2020/8884070