Dissecting genomic hotspots underlying seed protein, oil, and sucrose content in an interspecific mapping population of soybean using high‐density linkage mapping

• Published in: Plant biotechnology journal Vol. 16; no. 11; pp. 1939 - 1953
• Main Authors: Patil, Gunvant, Vuong, Tri D., Kale, Sandip, Valliyodan, Babu, Deshmukh, Rupesh, Zhu, Chengsong, Wu, Xiaolei, Bai, Yonghe, Yungbluth, Dennis, Lu, Fang, Kumpatla, Siva, Shannon, J. Grover, Varshney, Rajeev K., Nguyen, Henry T.
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.11.2018; John Wiley and Sons Inc
• Subjects: allelic variation; Analysis; bin map; biotechnology; Chromosome Mapping - methods; Domestication; Gene loci; Gene mapping; Genes; Genetic aspects; genetic background; Genome, Plant - genetics; Genomes; genomic hotspot; Genomics; genomic‐wide association study; Genotype & phenotype; Glycine max; Glycine max - genetics; Glycine max - metabolism; Glycine soja; Haplotypes; inbred lines; Inbreeding; Interspecific; oils; Oilseeds; Peptide mapping; Plant Proteins - metabolism; Polymorphism, Single Nucleotide - genetics; Population studies; Proteins; Quantitative genetics; Quantitative Trait Loci; quantitative traits; Recombination; seed composition traits; Seeds - metabolism; Sequence Analysis, DNA; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Soybean; soybean (Glycine max); Soybean Oil - metabolism; Soybeans; Sucrose; Sucrose - metabolism; Sugar; whole‐genome resequencing; genomic-wide association study; genomic hotspot; soybean (Glycine max); seed composition traits; quantitative trait loci; bin map; whole-genome resequencing
• ISSN: 1467-7644; 1467-7652; 1467-7652
• DOI: 10.1111/pbi.12929

Summary The cultivated [Glycine max (L) Merr.] and wild [Glycine soja Siebold & Zucc.] soybean species comprise wide variation in seed composition traits. Compared to wild soybean, cultivated soybean contains low protein, high oil, and high sucrose. In this study, an interspecific population was derived from a cross between G. max (Williams 82) and G. soja (PI 483460B). This recombinant inbred line (RIL) population of 188 lines was sequenced at 0.3× depth. Based on 91 342 single nucleotide polymorphisms (SNPs), recombination events in RILs were defined, and a high‐resolution bin map was developed (4070 bins). In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K‐SNP), derived from Illumina Infinium BeadChip sequencing platform. The QTL regions from both platforms were compared, and a significant concordance was observed between bin and 3K‐SNP markers. Importantly, the bin map derived from next‐generation sequencing technology enhanced mapping resolution (from 1325 to 50 Kb). A total of five, nine, and four QTLs were identified for protein, oil, and sucrose content, respectively, and some of the QTLs coincided with soybean domestication‐related genomic loci. The major QTL for protein and oil were mapped on Chr. 20 (qPro_20) and suggested negative correlation between oil and protein. In terms of sucrose content, a novel and major QTL were identified on Chr. 8 (qSuc_08) and harbours putative genes involved in sugar transport. In addition, genome‐wide association using 91 342 SNPs confirmed the genomic loci derived from QTL mapping. A QTL‐based haplotype using whole‐genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.