三黄占2号EMS诱变突变体库的构建与稻瘟病抗性初步评价

三黄占2号是国际公认、具有稻瘟病持久抗性的籼稻品种。为了获得用于三黄占2号稻瘟病持久抗性基因鉴定及机制研究的突变体,利用化学诱变剂EMS对三黄占2号进行诱变处理,创建突变体库,并对三黄占2号EMS突变体进行稻瘟病抗性评价。以成苗率50%为标准,筛选出最佳EMS诱变浓度为0.8%。从M2代起以单粒传方法进行突变体库的构建,获得了性状相对稳定的7 387份M4代突变体。广东阳江稻瘟病自然诱发鉴定结果表明,约有5.9%和5.4%的材料的叶瘟和穗瘟抗性分别发生了突变,并观察到多种抗性变化类型,从中筛选出300份突变体作进一步的研究。...

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Published in广东农业科学 Vol. 38; no. 16; pp. 1 - 3
Main Author 杨健源 张少红 姜先芽 黄显良 黄章慧 曾列先 李传瑛 HEI LEUNG 朱小源 刘斌
Format Journal Article
LanguageChinese
Published 广东省农科院植物保护研究所,广东广州,510640%广东省农科院水稻研究所,广东广州,510640%阳江市农业科学研究所,广东阳江,529500%国际水稻研究所,菲律宾马尼拉DAP0777 2011
Subjects
EMS
三黄占2号
稻瘟病抗性评价
突变体
稻瘟病抗性评价
EMS
突变体
三黄占2号
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ISSN1004-874X
DOI10.3969/j.issn.1004-874X.2011.16.001

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Summary:三黄占2号是国际公认、具有稻瘟病持久抗性的籼稻品种。为了获得用于三黄占2号稻瘟病持久抗性基因鉴定及机制研究的突变体,利用化学诱变剂EMS对三黄占2号进行诱变处理,创建突变体库,并对三黄占2号EMS突变体进行稻瘟病抗性评价。以成苗率50%为标准,筛选出最佳EMS诱变浓度为0.8%。从M2代起以单粒传方法进行突变体库的构建,获得了性状相对稳定的7 387份M4代突变体。广东阳江稻瘟病自然诱发鉴定结果表明,约有5.9%和5.4%的材料的叶瘟和穗瘟抗性分别发生了突变,并观察到多种抗性变化类型,从中筛选出300份突变体作进一步的研究。
Bibliography:44-1267/S
Sanhuangzhan 2（SHZ-2） is a well known indica rice variety with durable blast resistance.In order to develop the mutants for studying the mechanism of durable blast resistance,we produced a collection of SHZ-2 mutants by using chemical mutagenes EMS,and the evaluation of blast resistance for the collection was conducted.The optimal EMS-induced concentration of 0.8% was obtained based on 50% seedling survival.7 387 mutant lines had been advanced to M4 generation by single seed descent from M2.M4 mutants were evaluated in the Baisha blast nursery,Yangjiang,Guangdong,China.The results indicated that the mutation frequency of resistance were about 5.9% for leaf blast and 5.4% for panicle blast,respectively.Several kinds of resistant mutations were also observed.About 300 mutant lines which showed different blast resistances were selected for further study.
YANG Jian-yuan,ZHANG Shao-hong,JIANG Xian-ya,HUANG Xian-liang,HUANG Zhang-hui, ZENG Lie-xian,LI Chuan-ying,HEI LEUNG,ZHU Xiao-yuan,LIU Bin （1.Plant Prot
ISSN:1004-874X
DOI:10.3969/j.issn.1004-874X.2011.16.001