杨树PtoCIPK2 基因的克隆和表达分析

钙调磷酸酶B类似蛋白互作蛋白激酶（CIPKs）在植物生长发育和抗逆过程中发挥着重要作用.以2个月龄的杨树（Populus tomentosa Carr.cv ‘BJHR01’）幼苗总RNA反转录的cDNA为模板,克隆得到PtoCIPK2（GeneBank登录号：KF225478）基因全长cDNA序列.该基因包含1 395 bp开放读码框,编码464个氨基酸,预测蛋白分子量为52.8 ku,等电点为9.19.蛋白质结构分析表明,PtoCIPK2具有保守的蛋白激酶结构域和NAF结构域.Real-Time qRT-PCR分析表明,PtoCIPK2在杨树幼苗主根、茎和叶片中均有表达,在叶片中的表达量最...

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Published in广东农业科学 Vol. 40; no. 20; pp. 132 - 136
Main Author 张杰伟 管阳 李瑞芬 陈亚娟 朱丹 魏建华
Format Journal Article
LanguageChinese
Published 北京市农林科学院北京农业生物技术研究中心/农业基因资源与生物技术北京市重点实验室,北京,100097 2013
Subjects
PtoCIPK2
干旱胁迫
杨树
盐胁迫
蛋白激酶
蛋白激酶
PtoCIPK2
盐胁迫
干旱胁迫
杨树
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Summary:钙调磷酸酶B类似蛋白互作蛋白激酶（CIPKs）在植物生长发育和抗逆过程中发挥着重要作用.以2个月龄的杨树（Populus tomentosa Carr.cv ‘BJHR01’）幼苗总RNA反转录的cDNA为模板,克隆得到PtoCIPK2（GeneBank登录号：KF225478）基因全长cDNA序列.该基因包含1 395 bp开放读码框,编码464个氨基酸,预测蛋白分子量为52.8 ku,等电点为9.19.蛋白质结构分析表明,PtoCIPK2具有保守的蛋白激酶结构域和NAF结构域.Real-Time qRT-PCR分析表明,PtoCIPK2在杨树幼苗主根、茎和叶片中均有表达,在叶片中的表达量最高,约为其在茎中表达量的4倍.PtoCIPK2响应了NaC1（300 mmol/L）、干旱和ABA （200μmol/L）的胁迫.在NaCl和干旱胁迫12 h后,PtoCIPK2的表达量分别上调了28和8倍,推测该基因在响应高盐和干旱胁迫时起重要作用.
Bibliography:44-1267/S
ZHANG Jie-wei, GUAN Yang, LI Rui-fen, CHEN Ya-juan, ZHU Dan, WEI Jian-hua （Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences/ Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing 100097, China）
protein kinases; Populus tomentosa; PtoCIPK2; salt stress; dehydration
CBL-interacting protein kinases （CIPKs） play an important role in development and stress-resistant of plant. In this study, the full length cDNA of PtoC1PK2 （GeneBank Accession No. KF225478） was cloned from the cDNA library prepared from 2-month-old Populus tomentosa trees using RT-PCR technique. The CDS of PtoCIPK2 contained 1 395 nucleotide, encoding 464 amino acid with the molecular weight about 52.8 ku and PI of 9.19. Protein structure analysis showed that PtoCIPK2 contained conservative P-kinase and NAF domain. Real time quantitative RT-PCR showed that the expression of PtoCIPK2 was higher in leaves in 6-month-old trees, about 4 times than that of in stem. Mean
ISSN:1004-874X