水稻OsLEA2基因的克隆及表达分析
利用RT-PCR技术从水稻中克隆到OsLEA2的eDNA。序列分析表明，OsLEA2基因的读码框为312bp，编码一个由103个氨基酸残基组成的蛋白，富含Ala、Lys、Glu、Hjs、Val和衄，不含Asn、Cys、Phe和TW。OsLEA2蛋白的1-73位氨基酸残基形成LEA_I结构域。OsLEA2蛋白的二级结构有3个Ⅱ·螺旋构象区域，没有伸展的B-片层构象，为亲水蛋白。进化树分析表明OsLEA2属于第4组LEA蛋白的4A亚组，OsLEA2与4A亚组LEA蛋白的氨基酸一致性为29％-45％。实时定量liT-PCR分析表明表明OsLEA2基因在水稻的不同生长发育时期的不同组织中都能表达，在完...
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| Published in | 西南农业学报 Vol. 25; no. 3; pp. 743 - 749 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
重庆三峡学院生命科学与工程学院,重庆,404000
2012
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-4829 |
| DOI | 10.3969/j.issn.1001-4829.2012.03.001 |
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| Abstract | 利用RT-PCR技术从水稻中克隆到OsLEA2的eDNA。序列分析表明，OsLEA2基因的读码框为312bp，编码一个由103个氨基酸残基组成的蛋白，富含Ala、Lys、Glu、Hjs、Val和衄，不含Asn、Cys、Phe和TW。OsLEA2蛋白的1-73位氨基酸残基形成LEA_I结构域。OsLEA2蛋白的二级结构有3个Ⅱ·螺旋构象区域，没有伸展的B-片层构象，为亲水蛋白。进化树分析表明OsLEA2属于第4组LEA蛋白的4A亚组，OsLEA2与4A亚组LEA蛋白的氨基酸一致性为29％-45％。实时定量liT-PCR分析表明表明OsLEA2基因在水稻的不同生长发育时期的不同组织中都能表达，在完熟期的根和穗中，OsLEA2基因的表达明显升高。 |
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| AbstractList | 利用RT-PCR技术从水稻中克隆到OsLEA2的eDNA。序列分析表明，OsLEA2基因的读码框为312bp，编码一个由103个氨基酸残基组成的蛋白，富含Ala、Lys、Glu、Hjs、Val和衄，不含Asn、Cys、Phe和TW。OsLEA2蛋白的1-73位氨基酸残基形成LEA_I结构域。OsLEA2蛋白的二级结构有3个Ⅱ·螺旋构象区域，没有伸展的B-片层构象，为亲水蛋白。进化树分析表明OsLEA2属于第4组LEA蛋白的4A亚组，OsLEA2与4A亚组LEA蛋白的氨基酸一致性为29％-45％。实时定量liT-PCR分析表明表明OsLEA2基因在水稻的不同生长发育时期的不同组织中都能表达，在完熟期的根和穗中，OsLEA2基因的表达明显升高。 S511; 利用RT-PCR技术从水稻中克隆到OsLEA2的cDNA.序列分析表明,OsLEA2基因的读码框为312 bp,编码一个由103个氨基酸残基组成的蛋白,富含Ala、Lys、Glu、His、Val和Arg,不含Asn、Cys、Phe和Trp.OsLEA2蛋白的1～73位氨基酸残基形成LEA_1结构域.OsLEA2蛋白的二级结构有3个α-螺旋构象区域,没有伸展的β-片层相象,为素水蛋白.进化树分析表明OsLEA2 属于第4组LEA蛋白的4A亚组,OsLEA2与4A亚组LEA蛋白的氨基酸一致性为29％～45％.实时定量RT-PCR分析表明表明OsLEA2基因在水稻的不同生长发育时期的不同组织中都能表达,在完熟期的根和穗中,OsLEA2基因的表达明显升高. |
| Author | 胡廷章 吴应梅 杨俊年 吴晓丽 |
| AuthorAffiliation | 重庆三峡学院生命科学与工程学院,重庆404000 |
| AuthorAffiliation_xml | – name: 重庆三峡学院生命科学与工程学院,重庆,404000 |
| Author_FL | WU Ying-mei HU Ting-zhang YANG Jun-nian WU Xiao-li |
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| DocumentTitleAlternate | Molecular Cloning,Expression and Sequence Analysis of OsLEA2 Gene from Rice |
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| Keywords | OsLEA2 序列分析 基因表达 水稻 基因克隆 |
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| Notes | HU Ting-zhang, WU Ying-mei, YANG Jun-nian, WU Xiao-li （School of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404000, China） 51-1213/S The cDNA sequence of OsLEA2 from rice was cloned and analyzed by RT-PCR. The results showed that OsLEA2 contained a 312 bp ORF encoding a putative polypeptide of 103 amino acids, which was rich in Ala, Lys, Glu, His, Val and Arg, and lacking in Asu, Cys, Pbe and Trp residues. OsLEA2 protein contained a Pfam：LEA_I domain architecture at position 1 -73 with three a-helical domains and without {5-sheet domain, and was strongly hydrophilic. The phylogenetic relationship between related group 4 LEA proteins from different plants was analyzed, which showed that OsLEA2 belonged to subgroup 4A. The amino acid sequence of OsLEA2 showed 29 % -45 % se- quence identity with other members of subgroup 4A LEA proteins from plants. Real-time RT-PCR analysis revealed that OsLEA2 could ex- press in different tissue organ during different development stages of rice. I |
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| PublicationTitle | 西南农业学报 |
| PublicationTitleAlternate | Southwest China Journal of Agricultural Sciences |
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| SubjectTerms | OsLEA2 基因克隆 基因表达 序列分析 水稻 |
| Title | 水稻OsLEA2基因的克隆及表达分析 |
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