水稻OsLEA2基因的克隆及表达分析

利用RT-PCR技术从水稻中克隆到OsLEA2的eDNA。序列分析表明，OsLEA2基因的读码框为312bp，编码一个由103个氨基酸残基组成的蛋白，富含Ala、Lys、Glu、Hjs、Val和衄，不含Asn、Cys、Phe和TW。OsLEA2蛋白的1-73位氨基酸残基形成LEA_I结构域。OsLEA2蛋白的二级结构有3个Ⅱ·螺旋构象区域，没有伸展的B-片层构象，为亲水蛋白。进化树分析表明OsLEA2属于第4组LEA蛋白的4A亚组，OsLEA2与4A亚组LEA蛋白的氨基酸一致性为29％-45％。实时定量liT-PCR分析表明表明OsLEA2基因在水稻的不同生长发育时期的不同组织中都能表达，在完...

Full description

Saved in:
Bibliographic Details
Published in西南农业学报 Vol. 25; no. 3; pp. 743 - 749
Main Author 胡廷章 吴应梅 杨俊年 吴晓丽
Format Journal Article
LanguageChinese
Published 重庆三峡学院生命科学与工程学院,重庆,404000 2012
Subjects
OsLEA2
基因克隆
基因表达
序列分析
水稻
OsLEA2
序列分析
基因表达
水稻
基因克隆
Online AccessGet full text
ISSN1001-4829
DOI10.3969/j.issn.1001-4829.2012.03.001

Cover

More Information
Summary:利用RT-PCR技术从水稻中克隆到OsLEA2的eDNA。序列分析表明，OsLEA2基因的读码框为312bp，编码一个由103个氨基酸残基组成的蛋白，富含Ala、Lys、Glu、Hjs、Val和衄，不含Asn、Cys、Phe和TW。OsLEA2蛋白的1-73位氨基酸残基形成LEA_I结构域。OsLEA2蛋白的二级结构有3个Ⅱ·螺旋构象区域，没有伸展的B-片层构象，为亲水蛋白。进化树分析表明OsLEA2属于第4组LEA蛋白的4A亚组，OsLEA2与4A亚组LEA蛋白的氨基酸一致性为29％-45％。实时定量liT-PCR分析表明表明OsLEA2基因在水稻的不同生长发育时期的不同组织中都能表达，在完熟期的根和穗中，OsLEA2基因的表达明显升高。
Bibliography:HU Ting-zhang, WU Ying-mei, YANG Jun-nian, WU Xiao-li （School of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404000, China）
51-1213/S
The cDNA sequence of OsLEA2 from rice was cloned and analyzed by RT-PCR. The results showed that OsLEA2 contained a 312 bp ORF encoding a putative polypeptide of 103 amino acids, which was rich in Ala, Lys, Glu, His, Val and Arg, and lacking in Asu, Cys, Pbe and Trp residues. OsLEA2 protein contained a Pfam：LEA_I domain architecture at position 1 -73 with three a-helical domains and without {5-sheet domain, and was strongly hydrophilic. The phylogenetic relationship between related group 4 LEA proteins from different plants was analyzed, which showed that OsLEA2 belonged to subgroup 4A. The amino acid sequence of OsLEA2 showed 29 % -45 % se- quence identity with other members of subgroup 4A LEA proteins from plants. Real-time RT-PCR analysis revealed that OsLEA2 could ex- press in different tissue organ during different development stages of rice. I
ISSN:1001-4829
DOI:10.3969/j.issn.1001-4829.2012.03.001