适合MSAP分析的核桃子叶DNA提取方法

核桃（Juglans regiaL．）营养价值高，含有丰富的脂肪酸和蛋白质[1]，子叶是核桃重要的营养储存器官，作为常用的实验材料，可用来组织培养进行生根实验等相关研究[2-3]。MSAP是在AFLP基础上建立起来的在全基因组范围检测5’-CCGG位点的胞嘧啶甲基化变化的实验技术[4]，具有多态性高、无需预知DNA序列的优点，但其需要提取高质量的基因组DNA，存在于DNA中的多糖等杂质会对酶切等后续实验造成较大影响[5]。...

Full description

Saved in:
Bibliographic Details
Published in林业科学研究 Vol. 27; no. 1; pp. 128 - 132
Main Author 周贝贝 陈凌娜 张俊佩 裴东
Format Journal Article
LanguageChinese
Published 中国林业科学研究院林业研究所,林木遗传育种国家重点实验室,北京 100091 2014
Subjects
DNA提取
MSAP
多糖
子叶
核桃
MSAP
DNA提取
DNA extraction
polysaccharide
核桃
多糖
子叶
cotyledon
Juglans regia
Online AccessGet full text
ISSN1001-1498

Cover

More Information
Summary:核桃（Juglans regiaL．）营养价值高，含有丰富的脂肪酸和蛋白质[1]，子叶是核桃重要的营养储存器官，作为常用的实验材料，可用来组织培养进行生根实验等相关研究[2-3]。MSAP是在AFLP基础上建立起来的在全基因组范围检测5’-CCGG位点的胞嘧啶甲基化变化的实验技术[4]，具有多态性高、无需预知DNA序列的优点，但其需要提取高质量的基因组DNA，存在于DNA中的多糖等杂质会对酶切等后续实验造成较大影响[5]。
Bibliography:ZHOU Bei-bei, CHEN Ling-na ,ZHANG Jun-pei, PEI Dong （ Research Institute of Forestry, Chinese Academy of Forestry, State Key Laboratory of Tree Genetics and Breeding of State Forestry Administration, Beijing 100091, China）
11-1221/S
To obtain high-quality DNA suitable for methylation-sensitive amplification polymorphism （MSAP）, a- nalysis, the mature cotyledon and tissue cultured cotyledon of walnut eultivar ＇ Liaoning-1 ＇ were used as the testing materials in the research of DNA isolation by 3 methods （ SDS, high salt CTAB and modified high salt CTAB） and the mature leaf tissue was taken as the control. The concentration and intactness of the extracted DNA were deter- mined using a spectrometer and by electrophoresis in 0.8% （w/v） agarose gels against standard solutions of lambda DNA. High quality DNA was extracted from leaf tissues using the high salt CTAB method and the modified high salt CTAB method. No detectable DNA was extracted from the mature cotyledon and cultured cotyledon using SDS meth- od. The DNA
ISSN:1001-1498