Effect of bone marrow mesenchymal stem cell transplantation on acute hepatic failure in rats

• Published in: Experimental and therapeutic medicine Vol. 8; no. 4; pp. 1150 - 1158
• Main Authors: YUAN, SHUFANG, JIANG, TAO, ZHENG, RONGJIONG, SUN, LIHUA, CAO, GUIQIU, ZHANG, YUEXIN
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.10.2014; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: acute hepatic failure; Apoptosis; Bone marrow; bone mesenchymal stem cells; Care and treatment; cluster of differentiation 163; Cytokines; Interleukin-10; Liver; Liver failure; Mortality; Pathogenesis; Physiological aspects; rat model; Rodents; Standard deviation; Stem cell transplantation; Stem cells; Studies; Transplantation; Veins & arteries; China; United States > US; rat model; cluster of differentiation 163; acute hepatic failure; interleukin 10; bone mesenchymal stem cells
• ISSN: 1792-0981; 1792-1015
• DOI: 10.3892/etm.2014.1848

The aim of the present study was to investigate the effectiveness of bone marrow mesenchymal stem cell (BMSC) transplantation in the treatment of acute hepatic failure (AHF) in rats. BMSCs were isolated from rat bone marrow, cultured and analyzed by flow cytometry. Following BMSC transplantation into rats with AHF, the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB), direct bilirubin (DBIL) and indirect bilirubin (IBIL) in the serum were measured using an automatic biochemical analyzer. Hematoxylin and eosin (H&E) staining and a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were performed to analyze the pathological changes and apoptosis rate. Levels of cluster of differentiation (CD)163 and interleukin (IL)-10 in the serum and liver tissue were detected by an enzyme-linked immunosorbent assay (ELISA) assay and western blot analysis. Compared with the levels in the control group, the serum levels of ALT, AST, DBIL, IBIL, CD163 and IL-10 in the BMSC transplantation groups were significantly lower at 120 and 168 h, while the serum levels of ALB were significantly higher at 168 h after BMSC transplantation. The pathological features of liver failure were alleviated by BMSC transplantation. The expression levels of CD163 and IL-10 in the liver tissue were also significantly decreased following transplantation. The results indicate that BMSCs have a therapeutic effect on AHF in rats, and CD163 and IL-10 may be used as sensitive serum prognosis indicators in the early assessment of patients following liver transplantation.