Transcriptome Analysis of Redox Systems and Polyamine Metabolic Pathway in Hepatoma and Non-Tumor Hepatocyte-like Cells

• Published in: Biomolecules (Basel, Switzerland) Vol. 13; no. 4; p. 714
• Main Authors: Ivanova, Olga N, Krasnov, George S, Snezhkina, Anastasiya V, Kudryavtseva, Anna V, Fedorov, Vyacheslav S, Zakirova, Natalia F, Golikov, Michail V, Kochetkov, Sergey N, Bartosch, Birke, Valuev-Elliston, Vladimir T, Ivanov, Alexander V
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.04.2023; MDPI
• Subjects: Antibodies; antioxidant enzymes; Biochemistry, Molecular Biology; Biosynthesis; Carcinoma, Hepatocellular - genetics; Cells; Development and progression; Enzymes; Fibrosis; Gene expression; Gene Expression Profiling; Genes; Genetic aspects; Genomics; Health aspects; HepaRG; Hepatitis; Hepatocytes; Hepatocytes - metabolism; Hepatoma; Human health and pathology; Humans; Hépatology and Gastroenterology; Life Sciences; Liver cancer; Liver cells; Liver Neoplasms - genetics; Metabolic Networks and Pathways; Metabolic pathways; Metabolism; Molecular biology; Oxidation-Reduction; Oxidation-reduction reaction; Oxidative stress; Polyamines; Polyamines - metabolism; Post-translation; Progenitor cells; Proline - metabolism; Proteins; Reactive oxygen species; Reactive Oxygen Species - metabolism; RNA; Transcriptomes; Transcriptomics; Tumors; Urea; urea cycle; Russia; St Louis Missouri; United States > US; HepaRG; antioxidant enzymes; proline metabolism; urea cycle; polyamines; reactive oxygen species
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom13040714

Reactive oxygen species (ROS) play a major role in the regulation of various processes in the cell. The increase in their production is a factor contributing to the development of numerous pathologies, including inflammation, fibrosis, and cancer. Accordingly, the study of ROS production and neutralization, as well as redox-dependent processes and the post-translational modifications of proteins, is warranted. Here, we present a transcriptomic analysis of the gene expression of various redox systems and related metabolic processes, such as polyamine and proline metabolism and the urea cycle in Huh7.5 hepatoma cells and the HepaRG liver progenitor cell line, that are widely used in hepatitis research. In addition, changes in response to the activation of polyamine catabolism that contribute to oxidative stress were studied. In particular, differences in the gene expression of various ROS-producing and ROS-neutralizing proteins, the enzymes of polyamine metabolisms and proline and urea cycles, as well as calcium ion transporters between cell lines, are shown. The data obtained are important for understanding the redox biology of viral hepatitis and elucidating the influence of the laboratory models used.