Regulatory Networks in Seeds Integrating Developmental, Abscisic Acid, Sugar, and Light Signaling

• Published in: Plant physiology (Bethesda) Vol. 131; no. 1; pp. 78 - 92
• Main Authors: Inès M. Brocard-Gifford, Lynch, Tim J., Finkelstein, Ruth R.
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Biologists 01.01.2003; American Society of Plant Physiologists
• Subjects: abscisic acid; Abscisic Acid - pharmacology; Agronomy. Soil science and plant productions; Arabidopsis; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis Proteins; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Basic-Leucine Zipper Transcription Factors; biochemical pathways; Biological and medical sciences; Carbohydrate Metabolism; Carbohydrates; Carbohydrates - pharmacology; CCAAT-Enhancer-Binding Proteins; CCAAT-Enhancer-Binding Proteins - genetics; CCAAT-Enhancer-Binding Proteins - metabolism; chemical constituents of plants; Development and Hormone Action; DNA-binding proteins; drug effects; Economic plant physiology; embryo (plant); Embryogenesis; Embryonic growth stage; Fructification and ripening; Fructification, ripening. Postharvest physiology; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Developmental; Gene Expression Regulation, Developmental - drug effects; Gene Expression Regulation, Developmental - radiation effects; Gene Expression Regulation, Plant; Gene Expression Regulation, Plant - drug effects; Gene Expression Regulation, Plant - radiation effects; Genes; Genetic loci; Genetic mutation; genetics; Germination; Germination - drug effects; Germination - physiology; Germination - radiation effects; glucose; growth & development; Growth and development; Light; metabolism; mutants; Mutation; nucleotide sequences; pharmacology; physiology; Pigments, Biological; Pigments, Biological - metabolism; plant morphology; Plant physiology and development; plant proteins; Plant Proteins - genetics; Plant Proteins - metabolism; Plants; radiation effects; Regulatory Sequences, Nucleic Acid; Regulatory Sequences, Nucleic Acid - genetics; Saccharomyces cerevisiae; seed development; seed germination; Seedlings; Seeds; Seeds - genetics; Seeds - growth & development; Signal Transduction; Signal Transduction - drug effects; Signal Transduction - physiology; Signal Transduction - radiation effects; Starches; Sugar; Sugars; transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Two-Hybrid System Techniques; Vegetative and sexual reproduction, floral biology, fructification; Yeasts; Seeds; Germination; Arabidopsis thaliana; Phenotype; Regulation(control); Cruciferae; Dicotyledones; Angiospermae; Development; Spermatophyta; Mutation; Experimental plant; Transcription factor
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.011916

Progression through embryogenesis and the transition to germination is subject to regulation by many transcription factors, including those encoded by the Arabidopsis LEC1 (LEAFY COTYLEDON1), FUS3 (FUSCA3), and abscisic acid-insensitive (ABI) ABI3, ABI4, and ABI5 loci. To determine whether the ABI4, ABI5, LEC1, and FUS3 loci interact or act independently, we analyzed abi fus3 and abi lec1 double mutants. Our results show that both ABI4 and ABI5 interact genetically with both LEC1 and FUS3 in controlling pigment accumulation, suppression of vivipary, germination sensitivity to abscisic acid, gene expression during mid- and late embryogenesis, sugar metabolism, sensitivity to sugar, and etiolated growth. However, the relative strengths of the observed interactions vary among responses and may even be antagonistic. Furthermore, the interactions reveal cryptic effects of individual loci that are not detectable by analyses of single mutants. Despite these strong genetic interactions, but consistent with the disparities in peak expression of these loci, none of the ABI transcription factors appear to interact directly with either FUS3 or LEC1 in a yeast (Saccharomyces cerevisiae) two-hybrid assay system.