Ethanol responsive lnc171 promotes migration and invasion of HCC cells via mir-873-5p/ZEB1 axis

• Published in: BMC cancer Vol. 24; no. 1; p. 550
• Main Authors: Huang, Shiping, Liao, Zhouxiang, He, Xiao, Song, Zhenyu, Fang, Xi, Wen, Sha, Yang, Lichao, Li, Hui, Zhang, Qi, Mo, Wanling, Cheng, Xiaojing, He, Min, Huang, Xuejing
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 01.05.2024; BioMed Central; BMC
• Subjects: Acetaldehyde; Alcohol; Alcohol, Denatured; Analysis; Antibodies; Binding sites; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Care and treatment; Cell culture; Cell Line, Tumor; Cell Movement - genetics; ceRNA; Chromosome 5; Computational biology; Diagnosis; Ethanol; Ethanol - pharmacology; Gene Expression Regulation, Neoplastic; Genes; Health aspects; Hepatocellular carcinoma; Hepatoma; Homeobox; Humans; Invasion; Journalists; Liver cancer; Liver Neoplasms - genetics; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; lncRNA; Luciferase; Medical prognosis; MicroRNAs; MicroRNAs - genetics; Migration; Molecular modelling; Neoplasm Invasiveness - genetics; Non-coding RNA; Polymerase chain reaction; Proteins; Risk factors; RNA; RNA sequencing; RNA, Long Noncoding - genetics; siRNA; Software; Zinc Finger E-box-Binding Homeobox 1 - genetics; Zinc Finger E-box-Binding Homeobox 1 - metabolism; China; United States > US; ceRNA; Hepatocellular carcinoma; lncRNA; Ethanol; Migration; Invasion
• ISSN: 1471-2407; 1471-2407
• DOI: 10.1186/s12885-024-12309-3

Long nonconding RNAs (lncRNAs) have been found to be a vital regulatory factor in the development process of human cancer, and could regarded as diagnostic or prognostic biomarkers for human cancers. Here, we aim to confirm the expression and molecular mechanism of RP11-171K16.5 (lnc171) in hepatocellular carcinoma (HCC). Screening of differentially expressed lncRNAs by RNA sequencing. Expression level of gene was studied by quantitative real-time PCR (qRT-PCR). The effects of lnc171, mir-873-5p, and ethanol on migration and invasion activity of cells were studied used transwell assay, and luciferase reporter assay was used to confirm the binding site. RNA sequencing showed that lnc171 was markedly up-regulated in HCC. siRNA-mediated knockdown of lnc171 repressed the migration and invasion ability of HCC cells. Bioinformatic analysis, dual luciferase reporter assay, and qRT-PCR indicated that lnc171 interacted with mir-873-5p in HCC cells, and Zin-finger E-box binding homeobox (ZEB1) was a downstream target gene of mir-873-5p. In addition, lnc171 could enhance migration and invasion ability of HCC cells by up-regulating ZEB1 via sponging mir-873-5p. More interestingly, ethanol stimulation could up-regulate the increase of lnc171, thereby regulating the expression of competing endogenous RNA (ceRNA) network factors which lnc171 participated in HCC cells. Our date demonstrates that lnc171 was a responsive factor of ethanol, and plays a vital role in development of HCC via binding of mir-873-5p.