Reduced Proteolysis of Secreted Gelatin and Yps1-Mediated α-Factor Leader Processing in a Pichia pastoris kex2 Disruptant

• Published in: Applied and Environmental Microbiology Vol. 71; no. 5; pp. 2310 - 2317
• Main Authors: WERTEN, Marc W. T, DE WOLF, Frits A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.05.2005
• Subjects: active-site; Aspartic Acid Endopeptidases - physiology; aspartic protease 3; Base Sequence; Biodegradation; Biological and medical sciences; Biotechnology; Cloning, Molecular; Enzymes; Fundamental and applied biological sciences. Psychology; Fungal Proteins - physiology; Gelatin - metabolism; Genes; Genetic engineering; Genetic technics; in-vivo; kex2 protease; Mating Factor; Methods. Procedures. Technologies; Modification of gene expression level; Molecular Sequence Data; Peptides - metabolism; Physiology and Biotechnology; Pichia - metabolism; Pichia pastoris; Proprotein Convertases - physiology; Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins - physiology; schizosaccharomyces-pombe; yapsin 1 yap3p; Yeast; Fungi; Gelatin; Proteolysis; Secretion; Ascomycetes; Mutation; Pichia pastoris; Recombinant protein; Thallophyta
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.71.5.2310-2317.2005

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