ROS-Responsive Chitosan Coated Magnetic Iron Oxide Nanoparticles as Potential Vehicles for Targeted Drug Delivery in Cancer Therapy

• Published in: International journal of nanomedicine Vol. 15; pp. 3333 - 3346
• Main Authors: Ayyanaar, Srinivasan, Balachandran, Chandrasekar, Bhaskar, Rangaswamy Chinnabba, Kesavan, Mookkandi Palsamy, Aoki, Shin, Raja, Ramachandran Palpandi, Rajesh, Jegathalaprathaban, Webster, Thomas J, Rajagopal, Gurusamy
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2020; Dove; Dove Medical Press
• Subjects: 5-fluorouracil; Antineoplastic Agents - pharmacology; Antineoplastic Agents - therapeutic use; Biomedical engineering; Cancer; Cell Death - drug effects; Cell Line, Tumor; Chemotherapy; chitosan; Chitosan - chemistry; Complications and side effects; cytotoxicity; Drug Carriers - chemistry; Drug Delivery Systems; Drug Liberation; Drug therapy; Drugs; Ferric Compounds - chemistry; Ferric oxide; Fluorouracil - pharmacology; Fluorouracil - therapeutic use; Humans; Hydrogen-Ion Concentration; Influenza; Inhibitory Concentration 50; Iron compounds; magnetic iron oxide nanoparticles; Magnetite Nanoparticles - chemistry; Magnetite Nanoparticles - ultrastructure; Monounsaturated fatty acids; Nanoparticles; Neoplasms - drug therapy; Neoplasms - pathology; oleic acid; Oleic Acid - chemistry; Original Research; Particle Size; Reactive Oxygen Species - metabolism; Spectroscopy, Fourier Transform Infrared; targeted drug delivery; Temperature; X-Ray Diffraction; India; 5-fluorouracil; magnetic iron oxide nanoparticles; chitosan; targeted drug delivery; cytotoxicity; oleic acid
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/ijn.s249240

Cancer cells accumulate high concentrations of reactive oxygen species as a result of their faster and uninhibited metabolic activity. Cancer chemotherapeutic agents release an excess of severe adverse reactions as a result of targeting normal cells. This demands an improvement in targeted drug-delivery systems to selectively discharge anticancer drugs in the vicinity of such highly metabolically and mitotically active cells. Here, magnetic nanoparticles were synthesized by a traditional co-precipitation technique. Fe O @OA-CS-5-FLU-NPs were synthesized by an easy and rapid in situ loading method. The proposed Fe O @OA-CS-5-FLU-NPs were productively prepared as well as characterized by various spectroscopic and microscopic studies. The targeted drug release profile of the Fe O @OA-CS-5-FLU-NPs was studied in the presence of ROS including H O and pH induction. The released product, Fe O @OA-CS-5-FLU-NP, exhibited desirable levels of cytotoxicity and demonstrated morphological changes and inhibition of colony formation for A549 and HeLa S3 cancer cells. The IC50 values at 24 hours were 12.9 and 23 μg/mL, respectively. In summary, results from the MTT assay, fluorescence staining as well as colony formation assays, revealed that the Fe O @OA-CS-5-FLU-NPs were active and safe for anticancer biomedical applications. In summary, the present investigation provides a powerful nanostructured based system for improved cancer theranostics that should be further studied.