Therapeutic effects of various methods of MSC transplantation on cerebral resuscitation following cardiac arrest in rats

• Published in: Molecular medicine reports Vol. 13; no. 4; pp. 3043 - 3051
• Main Authors: LEONG, KA-HONG, ZHOU, LI-LI, LIN, QING-MING, WANG, PENG, YAO, LAN, HUANG, ZI-TONG
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.04.2016; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: Animals; Biomarkers; Blood Pressure; Blood-brain barrier; Bone marrow; bone marrow mesenchymal stem cells; Brain damage; Brain injury; Calcium-binding protein; Cardiac arrest; Care and treatment; Cell Culture Techniques; Cell Movement; Cell Separation; Cell Tracking; Cerebral blood flow; Cerebral ischemia; cerebral resuscitation; Cortex (temporal); CPR (First aid); Disease Models, Animal; Femur; global cerebral ischemic injury; Heart Arrest; Heart diseases; Heart Rate; Hippocampus - metabolism; Hippocampus - pathology; Humidity; Hypoxia; Immunophenotyping; Ischemia; Laboratory animals; Male; Mesenchymal Stem Cell Transplantation; Mesenchymal stem cells; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Mesenchyme; Mortality; Physiological aspects; Protein B; Proteins; Rats; Resuscitation - methods; Rodents; Serum levels; Standard deviation; Stem cell transplantation; Stem cells; Studies; Temporal Lobe - metabolism; Temporal Lobe - pathology; Transplantation; Ventricle; Ventricles (cerebral); Water content; China
• ISSN: 1791-2997; 1791-3004
• DOI: 10.3892/mmr.2016.4927

In the present study, mesenchymal stem cells (MSCs) were transplanted into the brain of rats following cardiopulmonary resuscitation (CPR) by three different methods: Direct stereotaxic injection into the lateral cerebral ventricle (LV), intra-carotid administration (A), and femoral venous infusion (V). The three different methods were compared by observing the effects of MSCs on neurological function following global cerebral hypoxia-ischemia, in order to determine the optimum method for MSC transplantation. MSCs were transplanted in groups A, V and LV following the restoration of spontaneous circulation. Neurological deficit scale scores were higher in the transplantation groups, as compared with the control group. Neuronal damage, brain water content and serum levels of S100 calcium-binding protein B were reduced in the hippo-campus and temporal cortex of the transplantation groups, as compared with the control rats following resuscitation. MSCs were able to migrate inside the brain tissue following transplantation, and were predominantly distributed in the hippocampus and temporal cortex where the neurons were vulnerable during global cerebral ischemia. These results suggest that transplantation of MSCs may notably improve neurological function following CPR in a rat model. Of the three different methods of MSC transplantation tested in the present study, LV induced the highest concentration of MSCs in brain areas vulnerable to global cerebral ischemia, and therefore, produced the best neurological outcome.