The Origin of the Multiline and g = 4.1 Electron Paramagnetic Resonance Signals from the Oxygen-Evolving System of Photosystem II

• Published in: Biophysical journal Vol. 51; no. 5; pp. 825 - 832
• Main Authors: Hansson, Örjan, Aasa, Roland, Vännga˙rd, Tore
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01.05.1987; Biophysical Society
• Subjects: Biological and medical sciences; Cell structures and functions; Chloroplast, photosynthetic membrane and photosynthesis; ESPECTROMETRIA; FOTOSINTESIS; Fundamental and applied biological sciences. Psychology; Molecular and cellular biology; OXIGENO; OXYGEN; OXYGENE; PHOTOSYNTHESE; PHOTOSYNTHESIS; SPECTROMETRIE; SPECTROMETRY; SPINACIA OLERACEA; Photosystem 2; Dicotyledones; Angiospermae; EPR spectrometry; Spermatophyta; Spinacia oleracea; Chenopodiaceae
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1016/S0006-3495(87)83409-4

Continuous illumination at 200 K of photosystem (PS) II-enriched membranes generates two electron paramagnetic resonance (EPR) signals that both are connected with the S 2 state: a multiline signal at g 2 and a single line at g = 4.1. From measurements at three different X-band frequencies and at 34 GHz, the g tensor of the multiline species was found to be isotropic with g = 1.982. It has an excited spin multiplet at ∼30 cm -1, inferred from the temperature-dependence of the linewidth. The intensity ratio of the g = 4.1 signal to the multiline signal was found to be almost constant from 5 to 23 K. Based on these findings and on spin quantitation of the two signals in samples with and without 4% ethanol, it is concluded that they arise from the ground doublets of paramagnetic species in different PS II centers. It is suggested that the two signals originate from separate PS II electron donors that are in a redox equilibrium with each other in the S 2 state and that the g = 4.1 signal arises from monomeric Mn(IV).