Supercritical carbon dioxide extraction of seed oil from yellow horn ( Xanthoceras sorbifolia Bunge.) and its anti-oxidant activity

• Published in: Bioresource technology Vol. 101; no. 7; pp. 2537 - 2544
• Main Authors: Zhang, Su, Zu, Yuan-Gang, Fu, Yu-Jie, Luo, Meng, Liu, Wei, Li, Ji, Efferth, Thomas
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.04.2010; [New York, NY]: Elsevier Ltd; Elsevier
• Subjects: analysis; Anti-oxidant activity; antioxidant activity; Antioxidants; Antioxidants - pharmacology; beta Carotene; beta Carotene - metabolism; Biological and medical sciences; Biotechnology; Biotechnology - methods; Biphenyl Compounds; Biphenyl Compounds - metabolism; carbon dioxide; Carbon Dioxide - chemistry; central composite design; chemical composition; chemistry; China; drug effects; fatty acid composition; Fatty Acids; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; isolation & purification; medicinal plants; medicinal properties; metabolism; methods; Particle Size; pharmacology; Picrates; Picrates - metabolism; Plant Oils; Plant Oils - chemistry; Plant Oils - isolation & purification; Plant Oils - pharmacology; Response surface methodology; Rheology; Rheology - drug effects; Sapindaceae; Sapindaceae - chemistry; Seed oil; seed oils; Seeds; Seeds - chemistry; Supercritical carbon dioxide extraction; supercritical fluid extraction; Surface Properties; Surface Properties - drug effects; Time Factors; unsaturated fatty acids; Xanthoceras sorbifolia; Yellow horn; China; Seed oil; Supercritical carbon dioxide extraction; Response surface methodology; Anti-oxidant activity; Yellow horn; Carbon dioxide; Response surface; Vegetable oil; Antioxidant; Supercritical state
• ISSN: 0960-8524; 1873-2976; 1873-2976
• DOI: 10.1016/j.biortech.2009.11.082

Supercritical fluid carbon dioxide (SF-CO 2) extraction (SFE) of seed oil from yellow horn and its anti-oxidant activity were investigated. The effects of CO 2 flow rate and particle size were firstly optimized, and a central composite design (CCD) combined with response surface methodology was used to study the effects of extraction pressure, temperature and time on the extraction yields. A maximal extraction yield of 61.28% was achieved under optimal conditions of extraction pressure 30 MPa at 45.68 °C, 2.08 h and CO 2 flow rate 12 kg/h with 0.5 mm particle size. By analyzing the chemical composition of the seed oil, we found that the content of unsaturated fatty acids was approximately 90%. Furthermore, the anti-oxidant activity of seed oil was assessed by a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay and a β-carotene bleaching test. Yellow horn seed oil possessed notable concentration-dependent anti-oxidant activity with IC 50 values of 0.151 and 0.195 g/mL, respectively.