Differences between femoral artery and vein smooth muscle cells in volume-regulated chloride channels

• Published in: Canadian journal of physiology and pharmacology Vol. 90; no. 11; pp. 1516 - 1526
• Main Authors: KANG, Xiao-Long, MIN ZHANG, JIE LIU, LV, Xiao-Fei, TANG, Yong-Bo, GUAN, Yong-Yuan
• Format: Journal Article
• Language: English
• Published: Plattsburgh, NY NRC Research Press 01.11.2012; National Research Council of Canada; Canadian Science Publishing NRC Research Press
• Subjects: Animals; Biological and medical sciences; Biological Transport; canaux chlorure régulés par le volume; Cell Line, Transformed; Cell Proliferation; Cells; Cells, Cultured; cellules de muscle lisse vasculaire; channels; Chloride channels; Chloride Channels - metabolism; Chlorides - metabolism; Comparative analysis; Femoral artery; Femoral Artery - cytology; Femoral Artery - metabolism; femoral artery and vein; Femoral Vein - cytology; Femoral Vein - metabolism; Fundamental and applied biological sciences. Psychology; Male; Membrane Potentials; modulation phénotypique; Muscle, Smooth, Vascular - cytology; Muscle, Smooth, Vascular - metabolism; Osmolar Concentration; Patch-Clamp Techniques; phenotypic modulation; Phosphorylation; Physiological aspects; Protein Kinase C - metabolism; Protein Processing, Post-Translational; Protein Tyrosine Phosphatases - metabolism; Protein-Tyrosine Kinases - metabolism; Proteins; Rats; Rats, Sprague-Dawley; Smooth muscle; smooth muscle cells; Vasodilation; veine et artère fémorales; Veins & arteries; Vertebrates: anatomy and physiology, studies on body, several organs or systems; volume-regulated Cl; China; Vertebrata; Femoral artery; Mammalia; Blood vessel; Chlorides; Smooth muscle; Ionic channel; Circulatory system; Vein; Cell volume
• ISSN: 0008-4212; 1205-7541
• DOI: 10.1139/y2012-117

The purpose of the present study was to compare the differences between the role of volume-regulated Cl – channels (VRCCs) in veins and arteries. We used the whole cell patch clamp and fluorescence imaging techniques to evaluate swelling-induced Cl – current (I Cl,vol ) and changes in the intracellular concentrations of Cl – ([Cl – ] i ) induced by hypotonic solutions in rat femoral artery cells (FASMCs) and vein smooth muscle cells (FVSMCs). I Cl,vol and [Cl – ] i decline induced by hypotonic solution were more prominent in FASMCs than in FVSMCs. I Cl,vol and the alterations in [Cl – ] i were gradually increased as the number of cell passages increased. However, the regulatory function of tyrosine protein phosphorylation in volume-regulated chloride movement is prominent in veins. The expression of ClC-3 was higher in FASMCs than in FVSMCs. VRCC activity is more pronounced in rat femoral arteries than in veins. VRCC activity and tyrosine protein phosphorylation regulative function increase gradually as vascular cells switch from contractile to proliferative phenotypes.