Overexpression of a modified 6-phosphofructo-1-kinase results in an increased itaconic acid productivity in Aspergillus niger

• Published in: AMB Express Vol. 3; no. 1; p. 57
• Main Authors: van der Straat, Laura, Tamayo-Ramos, Juan A, Schonewille, Tom, de Graaff, Leo H
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 13.09.2013; Springer
• Subjects: Aspergillus niger; Aspergillus terreus; Biomedical and Life Sciences; Biotechnology; Life Sciences; Microbial Genetics and Genomics; Microbiology; Original; Original Article; Systeem en Synthetische Biologie; Systems and Synthetic Biology; VLAG; aconitate decarboxylase; Itaconic acid; Mitochondrial transporter; Plasmamembrane transporter; Modified 6-phosphofructo-1-kinase
• ISSN: 2191-0855; 2191-0855
• DOI: 10.1186/2191-0855-3-57

A modified 6-phosphofructo-1-kinase was expressed in a citrate producing Aspergillus niger strain in combination with cis -aconitate decarboxylase from Aspergillus terreus to study the effect on the production of itaconic acid. The modified pfkA gene was also expressed in combination with the itaconic acid biosynthetic cluster from A. terreus , which consists of cis- aconitate decarboxylase cadA , a putative mitochondrial transporter mttA and a putative plasmamembrane transporter mfsA . The combined expression of pfkA and cadA resulted in increased citrate levels, but did not show increased itaconic acid levels. The combined expression of pfkA with the itaconic acid biosynthetic cluster resulted in significantly increased itaconic acid production at earlier time points. Also the itaconic acid productivity increased significantly. The maximum itaconic acid productivity that was reached under these conditions was 0.15 g/L/h, which is only a factor 17 lower than the 2.5 g/L/h that according to the US Department of Energy should be achieved to have an economically feasible production process.